@inbook{efdb15c901e847409374bc49ff192221,
title = "Isolation of human skin lymphatic endothelial cells and 3D reconstruction of the lymphatic vasculature in vitro",
abstract = "Studies of lymphangiogenesis and lymphatic endothelial biology in vitro require pure cultures of lymphatic endothelial cells and 3D vascular constructs, which closely resemble native human lymphatic vasculature. We describe a method for the isolation of human dermal microvascular lymphatic endothelial cells and generation of a 3D lymphatic capillary network. The lymphatic vascular construct is generated by coculturing primary lymphatic endothelial cells and fibroblasts in their native matrix, without the use of synthetic scaffolds or exogenous factors. The tissue is stable over many weeks and accurately recapitulates features of human dermal lymphatic microvasculature.",
keywords = "3D lymphatic capillary construct, Human lymphatic endothelial cells, Lymphangiogenesis in vitro, Lymphatic endothelial cell isolation, Lymphatic microvascular network in vitro, Self-assembly",
author = "Anita Rogic and Francois Auger and Mihaela Skobe",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8712-2_18",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "279--290",
booktitle = "Methods in Molecular Biology",
}