Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

José Ángel Nicolás-Ávila; María Sánchez-Diaz; Andrés Hidalgo

doi:10.1016/j.xpro.2020.100286

Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

José Ángel Nicolás-Ávila, María Sánchez-Diaz, Andrés Hidalgo

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020).

Original language	English
Article number	100286
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2020.100286
State	Published - 19 Mar 2021
Externally published	Yes

Keywords

Cell isolation
Flow cytometry/mass cytometry
Immunology

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10.1016/j.xpro.2020.100286

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title = "Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting",

abstract = "Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicol{\'a}s-{\'A}vila et al. (2020).",

keywords = "Cell isolation, Flow cytometry/mass cytometry, Immunology",

author = "Nicol{\'a}s-{\'A}vila, {Jos{\'e} {\'A}ngel} and Mar{\'i}a S{\'a}nchez-Diaz and Andr{\'e}s Hidalgo",

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AU - Nicolás-Ávila, José Ángel

AU - Sánchez-Diaz, María

AU - Hidalgo, Andrés

PY - 2021/3/19

Y1 - 2021/3/19

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AB - Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020).

KW - Cell isolation

KW - Flow cytometry/mass cytometry

KW - Immunology

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Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

Abstract

Keywords

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