TY - JOUR
T1 - Isolation of a lectin binding rhamnogalacturonan-I containing pectic polysaccharide from pumpkin
AU - Zhao, Jing
AU - Zhang, Fuming
AU - Liu, Xinyue
AU - St. Ange, Kalib
AU - Zhang, Anqiang
AU - Li, Quanhong
AU - Linhardt, Robert J.
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/5/1
Y1 - 2017/5/1
N2 - A rhamnogalacturonan-I (RG-I) containing pectic polysaccharide (PPc) was isolated from pumpkin following a low-temperature alkali treatment and a combination of gradual alcohol precipitation and ion-exchange. Monosaccharide compositional analysis of PPc revealed the presence of rhamnose, galacturonic acid, galactose, and arabinose in a molar ratio of 7.4: 25: 28: 2.6. Structural and linkage analysis by 1D NMR (1H NMR and 13C NMR), and 2D NMR (COSY, TOCSY, HSQC, and elevated temperature HMBC) suggested that PPc was a RG-I-like pectic polysaccharide, branched at the C-4 of some of the (about 29% of) rhamnosyl units, with relatively long β-1,4-D-galactan side chains to which were attached, through the C-3 of β-D-Gal, terminal non reducing α-Araf units. The results of surface plasmon resonance (SPR) show that PPc binds to two types of lectin, Ricinus communis agglutinin 120 (RCA120) and Galectin-3 (Gal-3). These binding studies show quick association and slow dissociation with a moderate binding affinity between PPc and Gal-3 of 1.26 μM. The interaction between PPc and Gal-3 suggest the potential use of pumpkin pectic polysaccharide as a Gal-3 inhibitor in functional food or drug development applications.
AB - A rhamnogalacturonan-I (RG-I) containing pectic polysaccharide (PPc) was isolated from pumpkin following a low-temperature alkali treatment and a combination of gradual alcohol precipitation and ion-exchange. Monosaccharide compositional analysis of PPc revealed the presence of rhamnose, galacturonic acid, galactose, and arabinose in a molar ratio of 7.4: 25: 28: 2.6. Structural and linkage analysis by 1D NMR (1H NMR and 13C NMR), and 2D NMR (COSY, TOCSY, HSQC, and elevated temperature HMBC) suggested that PPc was a RG-I-like pectic polysaccharide, branched at the C-4 of some of the (about 29% of) rhamnosyl units, with relatively long β-1,4-D-galactan side chains to which were attached, through the C-3 of β-D-Gal, terminal non reducing α-Araf units. The results of surface plasmon resonance (SPR) show that PPc binds to two types of lectin, Ricinus communis agglutinin 120 (RCA120) and Galectin-3 (Gal-3). These binding studies show quick association and slow dissociation with a moderate binding affinity between PPc and Gal-3 of 1.26 μM. The interaction between PPc and Gal-3 suggest the potential use of pumpkin pectic polysaccharide as a Gal-3 inhibitor in functional food or drug development applications.
KW - Lectin
KW - NMR
KW - Pectin
KW - Pumpkin residues
KW - Rhamnogalacturonan-I
UR - http://www.scopus.com/inward/record.url?scp=85010755086&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2017.01.067
DO - 10.1016/j.carbpol.2017.01.067
M3 - Article
C2 - 28267513
AN - SCOPUS:85010755086
SN - 0144-8617
VL - 163
SP - 330
EP - 336
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -