Isolation and characterization of cloned human fetal globin genes

Francesco Ramirez; A. Lee Burns; J. Gregory Mears; Sally Spence; Deborah Starkman; Arthur Bank

doi:10.1093/nar/7.5.1147

Isolation and characterization of cloned human fetal globin genes

Francesco Ramirez, A. Lee Burns, J. Gregory Mears, Sally Spence, Deborah Starkman, Arthur Bank

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Abstract

Three clones containing both the human ^Gγand ^Aγ globin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5′ most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the γ gene on the opposite chromosome. Restriction analysis of the three clones indicates that the ^Gγ and ^Aγ genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kb between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the γ genes in man.

Original language	English
Pages (from-to)	1147-1162
Number of pages	16
Journal	Nucleic Acids Research
Volume	7
Issue number	5
DOIs	https://doi.org/10.1093/nar/7.5.1147
State	Published - 10 Nov 1979
Externally published	Yes

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title = "Isolation and characterization of cloned human fetal globin genes",

abstract = "Three clones containing both the human Gγand Aγ globin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5′ most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the γ gene on the opposite chromosome. Restriction analysis of the three clones indicates that the Gγ and Aγ genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kb between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the γ genes in man.",

author = "Francesco Ramirez and Burns, {A. Lee} and Mears, {J. Gregory} and Sally Spence and Deborah Starkman and Arthur Bank",

note = "Funding Information: We thank Dr. Gek Kee Sim for many helpful discussion, Ms. Pamela Schwarsberg for her technical assistance and most of all Mr. Michael R. Stein for his patience and support. These studies were supported by NIH Grants GM-23143 and AM-25274 and NSF Grant 24120.",

year = "1979",

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doi = "10.1093/nar/7.5.1147",

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T1 - Isolation and characterization of cloned human fetal globin genes

AU - Ramirez, Francesco

AU - Burns, A. Lee

AU - Mears, J. Gregory

AU - Spence, Sally

AU - Starkman, Deborah

AU - Bank, Arthur

N1 - Funding Information: We thank Dr. Gek Kee Sim for many helpful discussion, Ms. Pamela Schwarsberg for her technical assistance and most of all Mr. Michael R. Stein for his patience and support. These studies were supported by NIH Grants GM-23143 and AM-25274 and NSF Grant 24120.

PY - 1979/11/10

Y1 - 1979/11/10

N2 - Three clones containing both the human Gγand Aγ globin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5′ most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the γ gene on the opposite chromosome. Restriction analysis of the three clones indicates that the Gγ and Aγ genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kb between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the γ genes in man.

AB - Three clones containing both the human Gγand Aγ globin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5′ most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the γ gene on the opposite chromosome. Restriction analysis of the three clones indicates that the Gγ and Aγ genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kb between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the γ genes in man.

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JO - Nucleic Acids Research

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Isolation and characterization of cloned human fetal globin genes

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