TY - JOUR
T1 - Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji
AU - Nyormoi, O.
AU - Sinclair, J. H.
AU - Klein, G.
N1 - Funding Information:
This work was supported by NSF Grant GB-19450 to J. H. S.; ACS Grant IN-46N to Indiana University; IU Doctoral StudentG rant-in-Aid, 10-430-32to 0. N.; contract No. NIH-NCl-E-69-2005, Grants from the Swedish Cancer Society and from the Damon Rynyon Memorial Fund for Cancer Research (DRG-1064-A) to G. K. This paper is Contribution No. 902 from the Departmento f Zoology, Indiana University.
PY - 1973/12
Y1 - 1973/12
N2 - A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.
AB - A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.
UR - https://www.scopus.com/pages/publications/0015750549
U2 - 10.1016/0014-4827(73)90337-6
DO - 10.1016/0014-4827(73)90337-6
M3 - Article
C2 - 4358110
AN - SCOPUS:0015750549
SN - 0014-4827
VL - 82
SP - 241
EP - 251
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -