Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji

O. Nyormoi; J. H. Sinclair; G. Klein

doi:10.1016/0014-4827(73)90337-6

Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji

O. Nyormoi
, J. H. Sinclair
, G. Klein

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10^-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.

Original language	English
Pages (from-to)	241-251
Number of pages	11
Journal	Experimental Cell Research
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(73)90337-6
State	Published - Dec 1973
Externally published	Yes

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@article{0b3be54689154542b1593669706d7590,

title = "Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji",

abstract = "A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15\% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.",

author = "O. Nyormoi and Sinclair, \{J. H.\} and G. Klein",

note = "Funding Information: This work was supported by NSF Grant GB-19450 to J. H. S.; ACS Grant IN-46N to Indiana University; IU Doctoral StudentG rant-in-Aid, 10-430-32to 0. N.; contract No. NIH-NCl-E-69-2005, Grants from the Swedish Cancer Society and from the Damon Rynyon Memorial Fund for Cancer Research (DRG-1064-A) to G. K. This paper is Contribution No. 902 from the Departmento f Zoology, Indiana University.",

year = "1973",

month = dec,

doi = "10.1016/0014-4827(73)90337-6",

language = "English",

volume = "82",

pages = "241--251",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

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T1 - Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji

AU - Nyormoi, O.

AU - Sinclair, J. H.

AU - Klein, G.

N1 - Funding Information: This work was supported by NSF Grant GB-19450 to J. H. S.; ACS Grant IN-46N to Indiana University; IU Doctoral StudentG rant-in-Aid, 10-430-32to 0. N.; contract No. NIH-NCl-E-69-2005, Grants from the Swedish Cancer Society and from the Damon Rynyon Memorial Fund for Cancer Research (DRG-1064-A) to G. K. This paper is Contribution No. 902 from the Departmento f Zoology, Indiana University.

PY - 1973/12

Y1 - 1973/12

N2 - A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.

AB - A substrate adherent, fibroblast-like cell line (Raji-A) has been isolated from a suspension culture of an established Burkitt lymphoma cell line (Raji). Except for the altered morphology associated with substrate attachment, Raji-A is identical to Raji with respect to karyotype, isozyme composition, susceptibility to Epstein-Barr virus (EBV) infection and inducibility of latent EBV. In order to facilitate fusion experiments with Raji-A, drug resistant variants were induced by treating cells with ethylmethane sulfonate followed by selection in growth medium which contains 8-azaguanine. Three clones (AGRO, AGR3 and AGR6) were found to be resistant to 50 μg/ml of 8-azaguanine. They had only 10-15% as much hypoxanthineguanine phosphoribosyltransferase activity as wild type cells and a low plating efficiency of 1-3 × 10-6 in the selective medium, HAT. Potential uses of these variants for studying EBV-lymphoblastoid cell interactions as well as human genetics by cell hybridization are discussed.

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DO - 10.1016/0014-4827(73)90337-6

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C2 - 4358110

AN - SCOPUS:0015750549

SN - 0014-4827

VL - 82

SP - 241

EP - 251

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Isolation and characterization of an adherent, 8-azaguanine resistant variant of the Burkitt lymphoma cell line, Raji

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