@article{8cf1e92ea92c47c5a47ed6d04d646e8e,
title = "Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart",
abstract = "Hearts of mice lacking Isl1, a LIM homeodomain transcription factor, are completely missing the outflow tract, right ventricle, and much of the atria. isl1 expression and lineage tracing of isl1 -expressing progenitors demonstrate that Isl1 is a marker for a distinct population of undifferentiated cardiac progenitors that give rise to the cardiac segments missing in isl1 mutants. Isl1 function is required for these progenitors to contribute to the heart. In isl1 mutants, isl1-expressing progenitors are progressively reduced in number, and FGF and BMP growth factors are downregulated. Our studies define two sets of cardiogenic precursors, one of which expresses and requires Isl1 and the other of which does not. Our results have implications for the development of specific cardiac lineages, left-right asymmetry, cardiac evolution, and isolation of cardiac progenitor cells.",
author = "Cai, {Chen Leng} and Xingqun Liang and Yunqing Shi and Chu, {Po Hsien} and Pfaff, {Samuel L.} and Ju Chen and Sylvia Evans",
note = "Funding Information: We would like to thank Phil Soriano, Eric Mercer, and David Anderson for their generosity in providing lacZ indicator mice and Tom Jessell, Yasuto Tanabe, and Chris William for their generosity in providing isl1-cre mice. Thank you to Xiaoxue Zhang for critical technical assistance. We would like to thank Karen Lettieri, in the Pfaff lab, for her expert technical assistance and generosity. We would also like to thank Julie Anderson and Kim Weldy for their tremendous work with our mouse colony. Thanks to a number of people who sent us cDNAs to make probes for RNA in situ, including Karen Lyons, James Cross, Andy McMahon, Gail Martin, Robert Maxson, and Benoit Bruneau. Thank you to Janet Rossant and Claudio Stern for technical advice on double in situ staining. Thanks to Ken Chien for his continued encouragement and critical reading of the manuscript. This work was supported by grants to S.E. from AHA and NIH, and an AHA fellowship awarded to C.-L.C.",
year = "2003",
month = dec,
doi = "10.1016/S1534-5807(03)00363-0",
language = "English",
volume = "5",
pages = "877--889",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "6",
}