Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice

Pablo Stringa, D. Romanin, N. Lausada, M. Machuca, J. C. Raimondi, A. Cabanne, M. Rumbo, G. Gondolesi

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC + TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC + TAC groups were able to decrease postreperfusion nitrites levels (P <.05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P <.05) and improving animal survival (P <.002) compared with TAC alone or the untreated group. The IPC + TAC group was the only one that showed significant improvement in lung histological analysis (P <.05). The TAC and IPC + TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.

Original languageEnglish
Pages (from-to)2480-2485
Number of pages6
JournalTransplantation Proceedings
Volume45
Issue number6
DOIs
StatePublished - Jul 2013
Externally publishedYes

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