TY - JOUR
T1 - Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
AU - Stringa, Pablo
AU - Romanin, D.
AU - Lausada, N.
AU - Machuca, M.
AU - Raimondi, J. C.
AU - Cabanne, A.
AU - Rumbo, M.
AU - Gondolesi, G.
N1 - Funding Information:
This work was partially funded by grant PICT 1799 from Agencia Nacional de Promoción de la Ciencia y la Tecnología (ANPCYT) , Argentina. P.S. and D.R. are fellows from the Argentinean National Research Council (CONICET). M.R. and G.G. are researchers from CONICET.
PY - 2013/7
Y1 - 2013/7
N2 - The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC + TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC + TAC groups were able to decrease postreperfusion nitrites levels (P <.05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P <.05) and improving animal survival (P <.002) compared with TAC alone or the untreated group. The IPC + TAC group was the only one that showed significant improvement in lung histological analysis (P <.05). The TAC and IPC + TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.
AB - The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC + TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC + TAC groups were able to decrease postreperfusion nitrites levels (P <.05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P <.05) and improving animal survival (P <.002) compared with TAC alone or the untreated group. The IPC + TAC group was the only one that showed significant improvement in lung histological analysis (P <.05). The TAC and IPC + TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.
UR - http://www.scopus.com/inward/record.url?scp=84882262539&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2013.02.113
DO - 10.1016/j.transproceed.2013.02.113
M3 - Article
C2 - 23953566
AN - SCOPUS:84882262539
SN - 0041-1345
VL - 45
SP - 2480
EP - 2485
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 6
ER -