TY - JOUR
T1 - Is the osteoclast calcium "receptor" a receptor-operated calcium channel?
AU - Bax, C. M.R.
AU - Shankar, V. S.
AU - Moonga, B. S.
AU - Huang, C. L.H.
AU - Zaidi, M.
N1 - Funding Information:
Acknowledgments: The studies were supported in part by Arthritis and Rheumatism Council, U.K. (MZ), Leverhulme Trust, U.K. (MZ), Sandoz Foundation for Gerontological Research, Switzerland (MZ) and Medical Research Council, U.K. (MZ). We are grateful to Professor Iain MacIntyre for advice.
PY - 1992/3/16
Y1 - 1992/3/16
N2 - Elevated extracellular calcium levels ([Ca2+]e) inhibit osteoclast function by elevating cytosolic free calcium levels ([Ca2+]i), presumably via the activation of a surface Ca2+ "receptor". It is unclear whether or not Ca2+-induced [Ca2+]i elevation involves the direct gating, by the putative "receptor", of a divalent cation channel. The results show that [Ca2+]i elevation in response to elevated [Ca2+]e comprises a distinct component of Ca2+ influx, the magnitude of which can be decreased and increased, respectively, by depolarising (100 mM-[K+]) and hyperpolarising (1 μM-[valinomycin]) the osteoclast membrane. In addition, activation of the putative Ca2+ "receptor" by elevated [Ca2+]e causes influx of the related divalent cation, magnesium (Mg2+). We suggest that Ca2+ influx induced by Ca2+ "receptor" activation is a major component of the observed [Ca2+]i response.
AB - Elevated extracellular calcium levels ([Ca2+]e) inhibit osteoclast function by elevating cytosolic free calcium levels ([Ca2+]i), presumably via the activation of a surface Ca2+ "receptor". It is unclear whether or not Ca2+-induced [Ca2+]i elevation involves the direct gating, by the putative "receptor", of a divalent cation channel. The results show that [Ca2+]i elevation in response to elevated [Ca2+]e comprises a distinct component of Ca2+ influx, the magnitude of which can be decreased and increased, respectively, by depolarising (100 mM-[K+]) and hyperpolarising (1 μM-[valinomycin]) the osteoclast membrane. In addition, activation of the putative Ca2+ "receptor" by elevated [Ca2+]e causes influx of the related divalent cation, magnesium (Mg2+). We suggest that Ca2+ influx induced by Ca2+ "receptor" activation is a major component of the observed [Ca2+]i response.
UR - https://www.scopus.com/pages/publications/0026526137
U2 - 10.1016/0006-291X(92)90527-R
DO - 10.1016/0006-291X(92)90527-R
M3 - Article
C2 - 1312836
AN - SCOPUS:0026526137
SN - 0006-291X
VL - 183
SP - 619
EP - 625
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -