TY - JOUR
T1 - Iron oxide core oil-in-water nanoemulsion as tracer for atherosclerosis MPI and MRI imaging
AU - Prévot, Geoffrey
AU - Kauss, Tina
AU - Lorenzato, Cyril
AU - Gaubert, Alexandra
AU - Larivière, Mélusine
AU - Baillet, Julie
AU - Laroche-Traineau, Jeanny
AU - Jacobin-Valat, Marie Josée
AU - Adumeau, Laurent
AU - Mornet, Stéphane
AU - Barthélémy, Philippe
AU - Duonor-Cérutti, Martine
AU - Clofent-Sanchez, Gisèle
AU - Crauste-Manciet, Sylvie
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11/5
Y1 - 2017/11/5
N2 - Purpose For early atherosclerosis imaging, magnetic oil-in-water nanoemulsion (NE) decorated with atheroma specific monoclonal antibody was designed for Magnetic Particle Imaging (MPI) and Magnetic Resonance Imaging (MRI). MPI is an emerging technique based on direct mapping of superparamagnetic nanoparticles which may advantageously complement MRI. Methods NE oily droplets were loaded with superparamagnetic iron oxide nanoparticles of 7, 11 and 18 nm and biofunctionalized with atheroma specific scFv-Fc TEG4-2C antibody. Results Inclusion of nanoparticles inside NE did not change the hydrodynamic diameter of the oil droplets, close to 180 nm, nor the polydispersity. The droplets were negatively charged (ζ = −30 mV). In vitro MPI signal was assessed by Magnetic Particle Spectroscopy (MPS). NE displayed MRI and MPS signals confirming its potential as new contrast agent. NE MPS signal increase with NPs size close to the gold standard (Resovist). In MRI, NE displayed R2* transversal relaxivity of 45.45, 96.04 and 218.81 mM−1 s−1 for 7, 11 and 18 nm respectively. NE selectively bind atheroma plaque both in vitro and ex vivo in animal models of atherosclerosis. Conclusion Magnetic NE showed reasonable MRI/MPS signals and a significant labelling of the atheroma plaque. These preliminary results support that NE platform could selectively image atherosclerosis.
AB - Purpose For early atherosclerosis imaging, magnetic oil-in-water nanoemulsion (NE) decorated with atheroma specific monoclonal antibody was designed for Magnetic Particle Imaging (MPI) and Magnetic Resonance Imaging (MRI). MPI is an emerging technique based on direct mapping of superparamagnetic nanoparticles which may advantageously complement MRI. Methods NE oily droplets were loaded with superparamagnetic iron oxide nanoparticles of 7, 11 and 18 nm and biofunctionalized with atheroma specific scFv-Fc TEG4-2C antibody. Results Inclusion of nanoparticles inside NE did not change the hydrodynamic diameter of the oil droplets, close to 180 nm, nor the polydispersity. The droplets were negatively charged (ζ = −30 mV). In vitro MPI signal was assessed by Magnetic Particle Spectroscopy (MPS). NE displayed MRI and MPS signals confirming its potential as new contrast agent. NE MPS signal increase with NPs size close to the gold standard (Resovist). In MRI, NE displayed R2* transversal relaxivity of 45.45, 96.04 and 218.81 mM−1 s−1 for 7, 11 and 18 nm respectively. NE selectively bind atheroma plaque both in vitro and ex vivo in animal models of atherosclerosis. Conclusion Magnetic NE showed reasonable MRI/MPS signals and a significant labelling of the atheroma plaque. These preliminary results support that NE platform could selectively image atherosclerosis.
KW - Atherosclerosis
KW - Human antibodies
KW - Iron oxide nanoparticles
KW - Magnetic particle imaging
KW - Nanoemulsion
UR - https://www.scopus.com/pages/publications/85029498943
U2 - 10.1016/j.ijpharm.2017.09.010
DO - 10.1016/j.ijpharm.2017.09.010
M3 - Article
C2 - 28899764
AN - SCOPUS:85029498943
SN - 0378-5173
VL - 532
SP - 669
EP - 676
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 2
ER -