Involvement of various combinations of endogenous inflammatory cytokines in Listeria monocytogenes-induced expression of inducible nitric oxide synthase in mice

Huabao Xiong, Takeaki Nishibori, Satoshi Ohya, Yoshinari Tanabe, Masao Mitsuyama

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Using an in vitro infection of spleen cells with Listeria monocytogenes, the relationship between endogenous cytokines and the expression of inducible nitric oxide synthase (iNOS) was examined. When all interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-1α, or the combination of IFN-γ with either TNF-α or IL-1α were neutralized by antibodies, there was a significant reduction of iNOS expression and nitrite production in culture. However, there was no reduction of iNOS expression and nitrite production when these cytokines were individually neutralized. After the depletion of natural killer cells, there was no change in the expression of Listeria-induced iNOS and nitrite production although the IFN-γ production was abrogated. Neutralization of TNF-α and IL-1α in natural killer cell-depleted culture resulted in the reduction of iNOS expression. Thus, various combinations of cytokines appeared to play an important role in iNOS induction by L. monocytogenes.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume16
Issue number3-4
DOIs
StatePublished - 31 Dec 1996
Externally publishedYes

Keywords

  • Listeria monocytogenes
  • cytokine
  • inducible nitric oxide synthase
  • natural killer cell

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