TY - JOUR
T1 - Involvement of various combinations of endogenous inflammatory cytokines in Listeria monocytogenes-induced expression of inducible nitric oxide synthase in mice
AU - Xiong, Huabao
AU - Nishibori, Takeaki
AU - Ohya, Satoshi
AU - Tanabe, Yoshinari
AU - Mitsuyama, Masao
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research (07670302,0 7307004)f rom the Ministry of Education, Science, Culture and Sports, and The Namba-Teruo Memorial Grant, Japan.
PY - 1996/12/31
Y1 - 1996/12/31
N2 - Using an in vitro infection of spleen cells with Listeria monocytogenes, the relationship between endogenous cytokines and the expression of inducible nitric oxide synthase (iNOS) was examined. When all interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-1α, or the combination of IFN-γ with either TNF-α or IL-1α were neutralized by antibodies, there was a significant reduction of iNOS expression and nitrite production in culture. However, there was no reduction of iNOS expression and nitrite production when these cytokines were individually neutralized. After the depletion of natural killer cells, there was no change in the expression of Listeria-induced iNOS and nitrite production although the IFN-γ production was abrogated. Neutralization of TNF-α and IL-1α in natural killer cell-depleted culture resulted in the reduction of iNOS expression. Thus, various combinations of cytokines appeared to play an important role in iNOS induction by L. monocytogenes.
AB - Using an in vitro infection of spleen cells with Listeria monocytogenes, the relationship between endogenous cytokines and the expression of inducible nitric oxide synthase (iNOS) was examined. When all interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-1α, or the combination of IFN-γ with either TNF-α or IL-1α were neutralized by antibodies, there was a significant reduction of iNOS expression and nitrite production in culture. However, there was no reduction of iNOS expression and nitrite production when these cytokines were individually neutralized. After the depletion of natural killer cells, there was no change in the expression of Listeria-induced iNOS and nitrite production although the IFN-γ production was abrogated. Neutralization of TNF-α and IL-1α in natural killer cell-depleted culture resulted in the reduction of iNOS expression. Thus, various combinations of cytokines appeared to play an important role in iNOS induction by L. monocytogenes.
KW - Listeria monocytogenes
KW - cytokine
KW - inducible nitric oxide synthase
KW - natural killer cell
UR - http://www.scopus.com/inward/record.url?scp=0030608458&partnerID=8YFLogxK
U2 - 10.1016/S0928-8244(96)00092-2
DO - 10.1016/S0928-8244(96)00092-2
M3 - Article
C2 - 9116644
AN - SCOPUS:0030608458
SN - 0928-8244
VL - 16
SP - 257
EP - 266
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 3-4
ER -