The cellular investigation of dopamine receptors was examined using glial cell fractions and synaptic fractions prepared from bovine caudate. Binding of the dopamine agonist apomorphine and antagonist spiroperidol was examined. A fractionation of bovine substantia nigra was developed. No evidence of haloperidol binding was found in neuronal, glial or synaptosomal fractions from this tissue. Finally, an autoradiographic localication of [3H]-haloperidol was undertaken. The results support the astroglial localization of a significant portion of the dopamine receptors.