Investigate global chromosomal interaction by Hi-C in human naive CD4 T cells

Xiangzhi Meng, Nicole Riley, Ryan Thompson, Siddhartha Sharma

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Biotin-streptavidin
  • Cell fixation
  • Chromosomal interaction
  • De novo ligation
  • Endo-restricted enzyme digestion
  • High-throughput sequencing


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