Investigate global chromosomal interaction by Hi-C in human naive CD4 T cells

Xiangzhi Meng, Nicole Riley, Ryan Thompson, Siddhartha Sharma

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-252
Number of pages14
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1712
ISSN (Print)1064-3745

Keywords

  • Biotin-streptavidin
  • Cell fixation
  • Chromosomal interaction
  • De novo ligation
  • Endo-restricted enzyme digestion
  • High-throughput sequencing

Fingerprint

Dive into the research topics of 'Investigate global chromosomal interaction by Hi-C in human naive CD4 T cells'. Together they form a unique fingerprint.

Cite this