Intraluminal middle cerebral artery occlusion (MCAO) model for ischemic stroke with laser doppler flowmetry guidance in mice

Saeed Ansari, Hassan Azari, Douglas J. McConnell, Aqeela Afzal, J. Mocco

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion in the United States in 20091,2. The intraluminal middle cerebral artery occlusion (MCAO) model was developed by Koizumi4 in 1986 to simulate this impactful human pathology in the rat. A modification of the MCAO method was later presented by Longa3. Both techniques have been widely used to identify molecular mechanisms of brain injury resulting from ischemic stroke and potential therapeutic modalities5. This relatively noninvasive method in rats has been extended to use in mice to take advantage of transgenic and knockout strains6,7. To model focal cerebral ischemia, an intraluminal suture is advanced via the internal carotid artery to occlude the base of the MCA. Retracting the suture after a specified period of time mimics spontaneous reperfusion, but the suture can also be permanently retained. This video will be demonstrating the two major approaches for performing intraluminal MCAO procedure in mice in a stepwise fashion, as well as providing insights for potential drawbacks and pitfalls. The ischemic brain tissue will subsequently be stained by 2,3,5-triphenyltetrazolium chloride (TTC) to evaluate the extent of cerebral infarction8.

Original languageEnglish
Article numbere2879
JournalJournal of Visualized Experiments
Issue number51
DOIs
StatePublished - May 2011
Externally publishedYes

Keywords

  • Cerebral ischemia
  • Intraluminal suture
  • Issue 51
  • Laser doppler
  • Medicine
  • Middle cerebral artery occlusion
  • Mouse

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