Intracellular Uncaging of cGMP with Blue Light

Hitesh K. Agarwal, Shenyu Zhai, D. James Surmeier, Graham C.R. Ellis-Davies

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We have made a new caged cGMP that is photolyzed with blue light. Using our recently developed derivative of 7-diethylaminocourmarin (DEAC) called DEAC450, we synthesized coumarin phosphoester derivatives of cGMP with two negative charges appended to the DEAC450 moiety. DEAC450-cGMP is freely soluble in physiological buffer without the need for any organic cosolvents. With a photolysis quantum yield of 0.18 and an extinction coefficient of 43 000 M-1 cm-1 at 453 nm, DEAC450-cGMP is the most photosensitive caged cGMP made to date. In patch-clamped neurons in acutely isolated brain slices, blue light effectively uncaged cGMP from DEAC450 and facilitated activation of hyperpolarization and cyclic nucleotide gated cation (HCN) channels in cholinergic interneurons. Thus, DEAC450-cGMP has a unique set of optical and chemical properties that make it a useful addition to the optical arsenal available to neurobiologists.

Original languageEnglish
Pages (from-to)2139-2144
Number of pages6
JournalACS Chemical Neuroscience
Issue number10
StatePublished - 18 Oct 2017


  • Caged compounds
  • HCN channels
  • blue light
  • cGMP
  • coumarin
  • neurophysiology


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