Intracarotid dehydrocholate infusion: A new method for prolonged reversible blood-brain barrier disruption

M. K. Spigelman, R. A. Zappulla, L. I. Malis, J. F. Holland, S. J. Goldsmith, J. D. Goldberg

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

An animal model for prolonged reversible blood-brain barrier (BBB) disruption has been developed. The external carotid arteries of Osborn-Mendel rats were catheterized in a retrograde manner. Varying concentrations of sodium dehydrocholate were infused into the internal carotid artery by this technique. BBB disruption was evaluated qualitatively by the appearance in the infused hemisphere of the systemically administered dyes Evans blue and sodium fluorescein and quantitatively by the ratio of counts of the technetium-labeled chelate of diethylenetriaminepentaacetic acid (99mTc-DTPA) in the infused to the noninfused hemisphere. The ability of sodium dehydrocholate to disrupt the BBB was documented with all three markers. As the concentration of the infused dehydrocholate was increased, both the incidence and the degree of BBB disruption increased. Reversibility of BBB disruption was evaluated by administration of sodium fluorescein and 99mTC-DTPA at varying times after BBB disruption. Depending on the concentration of the infused sodium dehydrocholate, altered BBB permeability can be maintained for over 3 days. This new model for prolonged reversible BBB disruption deserves further investigation both for basic studies of the BBB and for therapeutic studies of drug delivery into the central nervous system.

Original languageEnglish
Pages (from-to)606-612
Number of pages7
JournalUnknown Journal
Volume12
Issue number6
DOIs
StatePublished - 1983

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