@inbook{0496c944cd74419b847def4675f384b6,
title = "Interleukin-8 induces proliferation of ovarian cancer cells in 3D spheroids",
abstract = "Ovarian cancer (OC) is the most common cause of cancer deaths among gynecological malignancies. OC ascites contain multicellular spheroid aggregates, which exhibit increased pro-survival signaling, invasive behavior, and chemotherapeutic resistance. OC cells are characterized by an increased expression of the pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8), which increases their survival and migration, thus contributing to OC metastasis and angiogenesis. While previous studies have shown that IL-8 increases proliferation of OC cells grown in monolayer cultures, the effect of IL-8 on proliferation of OC cells grown in 3D spheroids has not been investigated. The spheroid 3D culture assays have been particularly useful in translational research since they allow cell-to-cell interactions that resemble tumor growth in vivo, while allowing easy cell manipulations and visualization. Here, we used the 3D spheroid culture assay to investigate the effect of IL-8 on OC cell proliferation. Using this assay, our results show that IL-8 significantly increases proliferation of OC cells grown in 3D spheroids.",
keywords = "3D culture, CXCL8, Cancer cell proliferation, Interleukin-8, Ovarian cancer, Spheroid formation",
author = "Uddin, {Mohammad M.} and Bijaya Gaire and Ivana Vancurova",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0247-8_10",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "117--124",
booktitle = "Methods in Molecular Biology",
}