Abstract
Interleukin-6 (IL-6) induction, as detected by bioassay and Northern analysis, was examined in vitro in endothelial cells or astrocytes derived from BALB/c (susceptible) or SJL (resistant) mice following exposure to mouse hepatitis virus (MHV-4) or UV inactivated MHV-4 (UV-MHV-4). In BALB/c endothelial cells, up to 16-fold more IL-6 (> 640 U/ml) was induced, compared to SJL cells which showed a minimal response (40 U/ml), relative to basal levels (< 20 U/ml). In contrast, both BALB/c and SJL astrocytes showed a substantial IL-6 response to MHV-4 and UV-MHV-4 exposure, although a strain difference persisted. Despite strain and cell specific differences in released IL-6, equivalent levels of IL-6 mRNA were induced in all cell types following exposure to MHV-4 or UV-MHV-4.
Original language | English |
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Pages (from-to) | 47-52 |
Number of pages | 6 |
Journal | Journal of Neuroimmunology |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1993 |
Externally published | Yes |
Keywords
- Astrocytes
- Bioassay
- Cerebral endothelial cells
- Interleukin-6
- Mouse hepatis virus (MHV-4, JHM)
- Northern analysis