Interferon γ selectively inhibits very primitive CD342+ CD38 and not more mature CD34+ CD38+ human hematopoietic progenitor cells

Hans Willem Snoeck, Dirk R.Van Bockstaele, Griet Nys, Marc Lenjou, Filip Lardon, Luc Haenen, Inez R. Odrigus, Marc E. Peetermans, Zwi N. Berneman

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

To assess the effects of interferon γ(IFN-γ) on very primitive hematopoietic progenitor cells, CD342+CD38 human bone marrow cells were isolated and cultured in a two-stage culture system, consisting of a primary liquid culture phase followed by a secondary semisolid colony assay. CD342+CD38 cells needed at least the presence of interleukin 3 (IL-3) and kit ligand (KL) together with either IL-1, IL-6, or granulocyte-colony-stimulating factor (G-CSF) in the primary liquid phase in order to proliferate and differentiate into secondary colony-forming cells (CFC). Addition of IFN-7 to the primary liquid cultures inhibited cell proliferation and generation of secondary CFC in a dose-dependent way. This was a direct effect since it was also seen in primary single cell cultures of CD342+ CD38 cells. The proliferation of more mature CD34+ CD38+ cells, however, was not inhibited by IFN-7, demonstrating for the first time that IFN-γ is a specific and direct hematopoietic stem cell inhibitor. IFN-γ, moreover, preserves the viability of CD342+CD38 cells in the absence of other cytokines. IFN-γ could, therefore, play a role in the protection of the stem cell compartment from exhaustion in situations of hematopoietic stress and may be useful as stem cell protecting agent against chemotherapy for cancer.

Original languageEnglish
Pages (from-to)1177-1182
Number of pages6
JournalJournal of Experimental Medicine
Volume180
Issue number3
DOIs
StatePublished - 1 Sep 1994
Externally publishedYes

Fingerprint

Dive into the research topics of 'Interferon γ selectively inhibits very primitive CD342+ CD38 and not more mature CD34+ CD38+ human hematopoietic progenitor cells'. Together they form a unique fingerprint.

Cite this