Interferon‐γ and interleukin‐4 reciprocally regulate the production of monocytes/macrophages and neutrophils through a direct effect on committed monopotential bone marrow progenitor cells

Hans‐W ‐W Snoeck, Filip Lardon, Marc Lenjou, Griet Nys, Dirk R. Van Bockstaele, Marc E. Peetermans

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24 Scopus citations

Abstract

We studied the direct effects of interferon‐γ (IFN‐γ) in single cell colony assays of CD34+HLA‐DR++ bone marrow progenitor cells stimulated by either granu‐locyte‐colony‐stimulating factor (G‐CSF), interleukin(IL)‐3, granulocyte/macro‐phage‐colony‐stimulating factor (GM‐CSF), combinations of these CSF or medium conditioned by the 5637 human bladder carcinoma cell line. In this culture system IFN‐γ stimulated monocytic colonies (CFU‐M) no matter which CSF or CSF combination was used to support them and inhibited granulocytic colonies (CFU‐G) if they were generated in the presence of G‐CSF. IL‐4 antagonized the myelopoietic effects of IFN‐γ: the IFN‐γ induced suppression of G‐CSF‐supported CFU‐G, as well as the stimulation of CFU‐M, were reversed by IL‐4. In all cultures, IFN‐γ had a limited, but statistically non‐significant, inhibitory effect on CFU‐GM, which was not affected by the presence of IL‐4. These data show that IFN‐γ and IL‐4 reciprocally regulate the generation of myeloid cells involved in humoral (neutrophils) and cellular (macrophages) immune responses through a direct effect on monopotential myeloid progenitor cells.

Original languageEnglish
Pages (from-to)1072-1077
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number5
DOIs
StatePublished - May 1993
Externally publishedYes

Keywords

  • CD34
  • Interferon‐γ
  • Interleukin‐4
  • Myelopoiesis
  • Progenitor cells

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