TY - JOUR
T1 - Intercellular adhesion molecule-1 induction
T2 - A sensitive and quantitative marker for cardiac allograft rejection
AU - Ohtani, Hiroshi
AU - Strauss, H. William
AU - Southern, James F.
AU - Tamatani, Takuya
AU - Miyasaka, Masayuki
AU - Sekiguchi, Morie
AU - Isobe, Mitsuaki
N1 - Funding Information:
From The First Department of Internal Medicine, Fukushima Medical College, Fukushima, Japan; *Division of Nuclear Medicine and Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts; and ?Pharmaceutical Basic Research Laboratories, Japan Tobacco Inc., Kanagawa, .~Departmcnt of Bioregulation Biomedical Research Center, Osaka University, Osaka and §First Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan. This investigation was supported in part by a grant from Bristol-Myers Squibb Pharmaceutical Co., Princeton, New Jersey. Fujisawa Pharmaceutical Co., Osaka, Japan supplied the FKS06 used in this study. Manuscript received July 22, 1994; revised manuscript received February. 14, 1995, accepted March 20, 1995. Address for correspondence: Dr. Mitsuaki Isobe, The First Department of Internal Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Mat-sumoto 390, Japan.
PY - 1995/9
Y1 - 1995/9
N2 - Objectives: Rats with abdominal heterotopic heart transplants were studied to determine whether cardiac allograft rejection could be assessed by immunoscintigraphy targeting intercellular adhesion molecule-1 (ICAM-1), which was induced on allografted organ cells in association with rejection. Background: It is important to detect early rejection before development of myocyte necrosis. Although a variety of methods for the detection of cardiac rejection have been investigated, histologic inspection of biopsied samples is still used routinely for clinical diagnosis of rejection. Methods: DA rat (RT-1a) hearts were transplanted into PVG rats (RT-1c). Immunohistologic examination of the allografts demonstrated that ICAM-1 induction on vascular endothelial cells was observed as early as 4 days after transplantation in this combination. Thirty-nine allografted rats and seven isografted rats were studied. One day after injection of 100 μCi of 111 Inlabeled anti-ICAM-1 monoclonal antibody (1A29), planar images were obtained. Results: Rejecting allografts showed increased radiotracer uptake and could be identified on the images as early as 5 days after transplantation. In contrast, nonrejecting cardiac allografts and isografts did not show specific uptake. Mildly rejecting allografts, with mononuclear cell infiltration but without significant myocyte necrosis, could be scintigraphically identified, and the level of radiotracer uptake reflected the histologic severity of rejection. Accumulation of 111In-labeled monoclonal antibody of isotypematched irrelevant specificity was not detected in the rejecting allografts. Conclusions: These data indicate that ICAM-1 induction can be assessed quantitatively by radioimmunoscintigraphy. Radioimmunoscintigraphy is a sensitive method for early detection and assessment of cardiac allograft rejection.
AB - Objectives: Rats with abdominal heterotopic heart transplants were studied to determine whether cardiac allograft rejection could be assessed by immunoscintigraphy targeting intercellular adhesion molecule-1 (ICAM-1), which was induced on allografted organ cells in association with rejection. Background: It is important to detect early rejection before development of myocyte necrosis. Although a variety of methods for the detection of cardiac rejection have been investigated, histologic inspection of biopsied samples is still used routinely for clinical diagnosis of rejection. Methods: DA rat (RT-1a) hearts were transplanted into PVG rats (RT-1c). Immunohistologic examination of the allografts demonstrated that ICAM-1 induction on vascular endothelial cells was observed as early as 4 days after transplantation in this combination. Thirty-nine allografted rats and seven isografted rats were studied. One day after injection of 100 μCi of 111 Inlabeled anti-ICAM-1 monoclonal antibody (1A29), planar images were obtained. Results: Rejecting allografts showed increased radiotracer uptake and could be identified on the images as early as 5 days after transplantation. In contrast, nonrejecting cardiac allografts and isografts did not show specific uptake. Mildly rejecting allografts, with mononuclear cell infiltration but without significant myocyte necrosis, could be scintigraphically identified, and the level of radiotracer uptake reflected the histologic severity of rejection. Accumulation of 111In-labeled monoclonal antibody of isotypematched irrelevant specificity was not detected in the rejecting allografts. Conclusions: These data indicate that ICAM-1 induction can be assessed quantitatively by radioimmunoscintigraphy. Radioimmunoscintigraphy is a sensitive method for early detection and assessment of cardiac allograft rejection.
UR - https://www.scopus.com/pages/publications/0029144089
U2 - 10.1016/0735-1097(95)00222-P
DO - 10.1016/0735-1097(95)00222-P
M3 - Article
C2 - 7642875
AN - SCOPUS:0029144089
SN - 0735-1097
VL - 26
SP - 793
EP - 799
JO - Journal of the American College of Cardiology
JF - Journal of the American College of Cardiology
IS - 3
ER -