Interactions of primary neuroepithelial progenitor and brain endothelial cells: Distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact

Miguel A.Gama Sosa, Rita De Gasperi, Anne B. Rocher, Gissel M. Perez, Keila Simons, Daniel E. Cruz, Patrick R. Hof, Gregory A. Elder

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Neurovascular interactions are crucial for the normal development of the central nervous system. To study such interactions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelial cell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favor maintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differentiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellular interactions needed for the proper development of the brain.

Original languageEnglish
Pages (from-to)619-626
Number of pages8
JournalCell Research
Volume17
Issue number7
DOIs
StatePublished - Jul 2007
Externally publishedYes

Keywords

  • Culture
  • Differentiation
  • Endothelial cells
  • Neuroepithelial cells

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