Interaction of heparin with annexin V

Ishan Capila, Victoria A. Vandernoot, Tanya R. Mealy, Barbara A. Seaton, Robert J. Linhardt

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51 Scopus citations

Abstract

The energetics and kinetics of the interaction of heparin with the Ca+2 and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca+2 dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a K(d) of ~21 nM for the interaction of annexin V with end-chain immobilized heparin and a K(d) of ~49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The K(d) of a heparin octasaccharide binding to annexin V was ~1 μM with a binding stoichiometry of 1:1. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalFEBS Letters
Volume446
Issue number2-3
DOIs
StatePublished - 12 Mar 1999
Externally publishedYes

Keywords

  • Annexin V
  • Heparin
  • Isothermal titration calorimetry
  • Octasaccharide
  • Surface plasmon resonance

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