Interaction cloning of NS1-I, a human protein that binds to the nonstructural NS1 proteins of influenza A and B viruses

Thorsten Wolff, Robert E. O'Neill, Peter Palese

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47 Scopus citations

Abstract

The yeast interaction trap system was used to identify NS1-I (for NS1 interactor), which is a human protein that hinds to the nonstructural NS1 protein of the influenza A virus. NS1-I is a human homolog of the porcine 17β-estradiol dehydrogenase precursor protein, to which it is 84% identical. We detected only one NS1-I mRNA species, of about 3.0 kb, in HeLa cells, and the NS1-I cDNA was found to have a coding capacity for a 79.6- kDa protein. However, immunoblot analysis detected predominantly a 55-kDa protein in human cells, suggesting that NS1-I, like the porcine 17β- estradiol dehydrogenase, is posttranslationally processed. Using an in vitro coprecipitation assay, we showed that NS1-I interacts with NS1 proteins from extracts of cells infected with five different influenza A virus strains as well as with the NS1 of an influenza B virus. The fact that influenza A and influenza B virus NS1 proteins bind to NS1-I suggests that this cellular protein plays a role in the influenza virus life cycle.

Original languageEnglish
Pages (from-to)5363-5372
Number of pages10
JournalJournal of Virology
Volume70
Issue number8
DOIs
StatePublished - Aug 1996

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