Integrin β1 subunit from Ostrinia furnacalis hemocytes: Molecular characterization, expression, and effects on the spreading of plasmatocytes

Jian Hu, Huafu Zhao, Xiaoqiang Yu, Jia Liu, Peng Wang, Jingya Chen, Qiuyun Xu, Wenqing Zhang

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

When lepidopteran larvae are infected by a large quantity of pathogens or parasitized by nonadaptive parasitoids, hemocytes in the hemocoel will encapsulate these foreign invaders. Cellular encapsulation requires hemocytes, particularly plasmatocytes, to change their states from nonadhesive, spherical cells into adhesive, spreading cells. However, it is unclear how the changes of plasmatocytes are regulated. Here we report that the integrin β1 subunit from hemocytes of Ostrinia furnacalis (Ofint β1) plays an important role in regulating the spreading of plasmatocytes. The full length cDNA sequence (4477. bp) of Ofint β1 was cloned from hemocytes. Phylogenetic analysis showed that Ofint β1 belonged to the integrin βPS family of Drosophila melanogaster with highest sequence identity (78.7%) to the β-integrin of Pseudoplusia includens. Structural analysis of the deduced amino acid sequence indicated that Ofint β1 had similar functional domains to known β-integrins in other lepidopteran insects. RT-PCR, Northern blotting, Western blotting and immunohistochemical analyses showed that OfINT β1 was expressed mainly in hemocytes, especially in plasmatocytes, and weakly in fat body, Malpighian tubes and epidermis. After hemocytes had spread onto slides, fewer antibodies to OfINT β1 bound to the surface of plasmatocytes. Furthermore, anti-. OfINT β1 serum clearly inhibited the spreading of plasmatocytes. Together these results indicate that OfINT β1 may play an important role in regulating the spreading of plasmatocytes.

Original languageEnglish
Pages (from-to)1846-1856
Number of pages11
JournalJournal of Insect Physiology
Volume56
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Keywords

  • Hemocytes
  • Integrin β
  • Ostrinia furnacalis
  • Plasmatocyte
  • Spreading

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