Integration site choice of a feline immunodeficiency virus vector

Yubin Kang; Christopher J. Moressi; Todd E. Scheetz; Litao Xie; Diane Thi Tran; Thomas L. Casavant; Prashanth Ak; Craig J. Benham; Beverly L. Davidson; Paul B. McCray

doi:10.1128/JVI.00719-06

Integration site choice of a feline immunodeficiency virus vector

Yubin Kang
, Christopher J. Moressi
, Todd E. Scheetz
, Litao Xie
, Diane Thi Tran
, Thomas L. Casavant
, Prashanth Ak
, Craig J. Benham
, Beverly L. Davidson
, Paul B. McCray

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a "bendable" structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

Original language	English
Pages (from-to)	8820-8823
Number of pages	4
Journal	Journal of Virology
Volume	80
Issue number	17
DOIs	https://doi.org/10.1128/JVI.00719-06
State	Published - Sep 2006
Externally published	Yes

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title = "Integration site choice of a feline immunodeficiency virus vector",

abstract = "We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21\% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a {"}bendable{"} structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.",

author = "Yubin Kang and Moressi, \{Christopher J.\} and Scheetz, \{Todd E.\} and Litao Xie and Tran, \{Diane Thi\} and Casavant, \{Thomas L.\} and Prashanth Ak and Benham, \{Craig J.\} and Davidson, \{Beverly L.\} and McCray, \{Paul B.\}",

year = "2006",

month = sep,

doi = "10.1128/JVI.00719-06",

language = "English",

volume = "80",

pages = "8820--8823",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "17",

}

TY - JOUR

T1 - Integration site choice of a feline immunodeficiency virus vector

AU - Kang, Yubin

AU - Moressi, Christopher J.

AU - Scheetz, Todd E.

AU - Xie, Litao

AU - Tran, Diane Thi

AU - Casavant, Thomas L.

AU - Ak, Prashanth

AU - Benham, Craig J.

AU - Davidson, Beverly L.

AU - McCray, Paul B.

PY - 2006/9

Y1 - 2006/9

N2 - We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a "bendable" structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

AB - We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a "bendable" structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

UR - https://www.scopus.com/pages/publications/33748639703

U2 - 10.1128/JVI.00719-06

DO - 10.1128/JVI.00719-06

M3 - Article

C2 - 16912328

AN - SCOPUS:33748639703

SN - 0022-538X

VL - 80

SP - 8820

EP - 8823

JO - Journal of Virology

JF - Journal of Virology

IS - 17

ER -

Integration site choice of a feline immunodeficiency virus vector

Abstract

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