TY - JOUR
T1 - Integration of Multiple Interferometers in Highly Multiplexed Diagnostic KITs to Evaluate Several Biomarkers of COVID-19 in Serum
AU - Murillo, Ana María M.
AU - Valle, Luis G.
AU - Ramírez, Yolanda
AU - Sánchez, María Jesús
AU - Santamaría, Beatriz
AU - Molina-Roldan, E.
AU - Ortega-Madueño, Isabel
AU - Urcelay, Elena
AU - Tramarin, Luca
AU - Herreros, Pedro
AU - Díaz-Perales, Araceli
AU - Garrido-Arandia, María
AU - Tome-Amat, Jaime
AU - Hernández-Ramírez, Guadalupe
AU - Espinosa, Rocío L.
AU - Laguna, María F.
AU - Holgado, Miguel
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/9
Y1 - 2022/9
N2 - In the present work, highly multiplexed diagnostic KITs based on an Interferometric Optical Detection Method (IODM) were developed to evaluate six Coronavirus Disease 2019 (COVID-19)-related biomarkers. These biomarkers of COVID-19 were evaluated in 74 serum samples from severe, moderate, and mild patients with positive polymerase chain reaction (PCR), collected at the end of March 2020 in the Hospital Clínico San Carlos, in Madrid (Spain). The developed multiplexed diagnostic KITs were biofunctionalized to simultaneously measure different types of specific biomarkers involved in COVID-19. Thus, the serum samples were investigated by measuring the total specific Immunoglobulins (sIgT), specific Immunoglobulins G (sIgG), specific Immunoglobulins M (sIgM), specific Immunoglobulins A (sIgA), all of them against SARS-CoV-2, together with two biomarkers involved in inflammatory disorders, Ferritin (FER) and C Reactive Protein (CRP). To assess the results, a Multiple Linear Regression Model (MLRM) was carried out to study the influence of IgGs, IgMs, IgAs, FER, and CRP against the total sIgTs in these serum samples with a goodness of fit of 73.01% (Adjusted R-Squared).
AB - In the present work, highly multiplexed diagnostic KITs based on an Interferometric Optical Detection Method (IODM) were developed to evaluate six Coronavirus Disease 2019 (COVID-19)-related biomarkers. These biomarkers of COVID-19 were evaluated in 74 serum samples from severe, moderate, and mild patients with positive polymerase chain reaction (PCR), collected at the end of March 2020 in the Hospital Clínico San Carlos, in Madrid (Spain). The developed multiplexed diagnostic KITs were biofunctionalized to simultaneously measure different types of specific biomarkers involved in COVID-19. Thus, the serum samples were investigated by measuring the total specific Immunoglobulins (sIgT), specific Immunoglobulins G (sIgG), specific Immunoglobulins M (sIgM), specific Immunoglobulins A (sIgA), all of them against SARS-CoV-2, together with two biomarkers involved in inflammatory disorders, Ferritin (FER) and C Reactive Protein (CRP). To assess the results, a Multiple Linear Regression Model (MLRM) was carried out to study the influence of IgGs, IgMs, IgAs, FER, and CRP against the total sIgTs in these serum samples with a goodness of fit of 73.01% (Adjusted R-Squared).
KW - COVID-19 biomarkers
KW - SARS-CoV-2
KW - ferritin
KW - immunoglobulin
KW - interferometric optical detection method
KW - serum
UR - https://www.scopus.com/pages/publications/85138727739
U2 - 10.3390/bios12090671
DO - 10.3390/bios12090671
M3 - Article
AN - SCOPUS:85138727739
SN - 2079-6374
VL - 12
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 671
ER -