Integration of genomic sequencing into the response to the Ebola virus outbreak in Nord Kivu, Democratic Republic of the Congo

Eddy Kinganda-Lusamaki, Allison Black, Daniel B. Mukadi, James Hadfield, Placide Mbala-Kingebeni, Catherine B. Pratt, Amuri Aziza, Moussa M. Diagne, Bailey White, Nella Bisento, Bibiche Nsunda, Marceline Akonga, Martin Faye, Ousmane Faye, Francois Edidi-Atani, Meris Matondo-Kuamfumu, Fabrice Mambu-Mbika, Junior Bulabula, Nicholas Di Paola, Matthias G. PauthnerKristian G. Andersen, Gustavo Palacios, Eric Delaporte, Amadou Alpha Sall, Martine Peeters, Michael R. Wiley, Steve Ahuka-Mundeke, Trevor Bedford, Jean Jacques Muyembe Tamfum

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

On 1 August 2018, the Democratic Republic of the Congo (DRC) declared its tenth Ebola virus disease (EVD) outbreak. To aid the epidemiologic response, the Institut National de Recherche Biomédicale (INRB) implemented an end-to-end genomic surveillance system, including sequencing, bioinformatic analysis and dissemination of genomic epidemiologic results to frontline public health workers. We report 744 new genomes sampled between 27 July 2018 and 27 April 2020 generated by this surveillance effort. Together with previously available sequence data (n = 48 genomes), these data represent almost 24% of all laboratory-confirmed Ebola virus (EBOV) infections in DRC in the period analyzed. We inferred spatiotemporal transmission dynamics from the genomic data as new sequences were generated, and disseminated the results to support epidemiologic response efforts. Here we provide an overview of how this genomic surveillance system functioned, present a full phylodynamic analysis of 792 Ebola genomes from the Nord Kivu outbreak and discuss how the genomic surveillance data informed response efforts and public health decision making.

Original languageEnglish
Pages (from-to)710-716
Number of pages7
JournalNature Medicine
Volume27
Issue number4
DOIs
StatePublished - Apr 2021
Externally publishedYes

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