Integrated genotype calling and association analysis of SNPs, common copy number polymorphisms and rare CNVs

Joshua M. Korn, Finny G. Kuruvilla, Steven A. McCarroll, Alec Wysoker, James Nemesh, Simon Cawley, Earl Hubbell, Jim Veitch, Patrick J. Collins, Katayoon Darvishi, Charles Lee, Marcia M. Nizzari, Stacey B. Gabriel, Shaun Purcell, Mark J. Daly, David Altshuler

Research output: Contribution to journalArticlepeer-review

639 Scopus citations

Abstract

Accurate and complete measurement of single nucleotide (SNP) and copy number (CNV) variants, both common and rare, will be required to understand the role of genetic variation in disease. We present Birdsuite, a four-stage analytical framework instantiated in software for deriving integrated and mutually consistent copy number and SNP genotypes. The method sequentially assigns copy number across regions of common copy number polymorphisms (CNPs), calls genotypes of SNPs, identifies rare CNVs via a hidden Markov model (HMM), and generates an integrated sequence and copy number genotype at every locus (for example, including genotypes such as A-null, AAB and BBB in addition to AA, AB and BB calls). Such genotypes more accurately depict the underlying sequence of each individual, reducing the rate of apparent mendelian inconsistencies. The Birdsuite software is applied here to data from the Affymetrix SNP 6.0 array. Additionally, we describe a method, implemented in PLINK, to utilize these combined SNP and CNV genotypes for association testing with a phenotype.

Original languageEnglish
Pages (from-to)1253-1260
Number of pages8
JournalNature Genetics
Volume40
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

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