@article{097e6b4217ca4d1bb3732c4878a46e0e,
title = "Intact Alzheimer amyloid precursor protein (APP) is present in platelet membranes and is encoded by platelet mRNA",
abstract = "Using antibodies directed against N-terminal and C-terminal epitopes we have immunologically detected APP species in the membrane and saline-soluble fractions of unstimulated platelets, and in the conditioned medium of thrombin-stimulated platelets. These studies demonstrate an intact 140kD membrane-associated form of APP that is released on degranulation. Evidence that platelets synthesize at least one form of APP (APP751) was obtained by enzymatic amplification of specific mRNA using Polymerase Chain Reaction (PCR) and direct sequence analysis of PCR product. Processing of APP for release may occur via successive C-terminal truncations, and/or by the release and proteolysis of an intact membrane associated form. An intact form of APP in platelets provides a circulating substrate upon which proteases from many tissues may act to produce beta protein (AB) during pathologic conditions.",
author = "Gardella, {J. E.} and J. Ghiso and Gorgone, {G. A.} and D. Marratta and Kaplan, {A. P.} and B. Frangione and Gorevic, {P. D.}",
note = "Funding Information: Acknowledements: Synthetic peptides SP18 and SP20 were synthesized in the Center for the Analysis and Synthesis of Macromolecules (CASM) at the State University of New York at Stony Brook (SUNY) by Ms. Karen Springer. This work was supported by grants from the American Heart Association, and the Center for Biotechnology at Stony Brook, and by AG05891, AG08721, AR02594, AG05891, and AG08721. We are grateful to Dr. Dmitry Goldgaber (Department of Psychiatry and Behavioral Science, SUNY Stony Brook) for his gift of cells expressing recombinant APP751, to Dr. Peter Newman (Department of Anatomy and Cellular biology, The Medical College of Wisconsin, Milwaukee, Wisconsin) for advice regarding the platelet mRNA studies, to Dr. Dennis Selkoe (Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical Center) for his gift of anti-C7 antiserum, and to Dr. Sesha Reddigari (Department of Medicine, SUNY Stony Brook) for his gift of Mab anti-hHMWK.",
year = "1990",
month = dec,
day = "31",
doi = "10.1016/S0006-291X(05)80927-1",
language = "English",
volume = "173",
pages = "1292--1298",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "3",
}