Insulin-induced expression of prostacyclin receptors on platelets is mediated through ADP-ribosylation of Giα protein

Nighat N. Kahn

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The binding of insulin in physiological amounts to human blood platelets, which increases adenylate cyclase-linked prostacyclin receptor numbers on the cell surface, was found to be directly related to the ADP- ribosylation of the Giα. Conversely, resuspension of the insulin-treated platelets in the hormone-free medium decreased both the prostaglandin receptor numbers and ADP-ribosylation of Giα. Furthermore, incubation of platelets with pertussis toxin or its A-protomer, which ADP-ribosylates Giα, also stimulated the binding of the prostanoid. These results suggest that the increase of prostacyclin receptor numbers in platelets is mediated through the ADP-ribosylation of Giα.

Original languageEnglish
Pages (from-to)2031-2038
Number of pages8
JournalLife Sciences
Volume63
Issue number22
DOIs
StatePublished - 23 Oct 1998

Keywords

  • ADP-ribosylation
  • Giα
  • Insulin
  • Platelets
  • Prostacyclin
  • Receptors

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