TY - JOUR
T1 - Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood
AU - Broxmeyer, Hal E.
AU - Luchsinger, Larry L.
AU - Weinberg, Rona Singer
AU - Jimenez, Alexandra
AU - Frenet, Emeline Masson
AU - van't Hof, Wouter
AU - Capitano, Maegan L.
AU - Hillyer, Christopher D.
AU - Kaplan, Mark H.
AU - Cooper, Scott
AU - Ropa, James
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023/11/21
Y1 - 2023/11/21
N2 - Umbilical cord blood transplantation is a life-saving treatment for malignant and non-malignant hematologic disorders. It remains unclear how long cryopreserved units remain functional, and the length of cryopreservation is often used as a criterion to exclude older units. We demonstrate that long-term cryopreserved cord blood retains similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved cord blood units. Long-term cryopreserved units contain highly functional cells, yielding robust engraftment in mouse transplantation models. We also leverage differences between units to examine gene programs associated with better engraftment. Transcriptomic analyses reveal that gene programs associated with lineage determination and oxidative stress are enriched in high engrafting cord blood, revealing potential molecular markers to be used as potency markers for cord blood unit selection regardless of length of cryopreservation. In summary, cord blood units cryopreserved for extended periods retain engrafting potential and can potentially be used for patient treatment.
AB - Umbilical cord blood transplantation is a life-saving treatment for malignant and non-malignant hematologic disorders. It remains unclear how long cryopreserved units remain functional, and the length of cryopreservation is often used as a criterion to exclude older units. We demonstrate that long-term cryopreserved cord blood retains similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved cord blood units. Long-term cryopreserved units contain highly functional cells, yielding robust engraftment in mouse transplantation models. We also leverage differences between units to examine gene programs associated with better engraftment. Transcriptomic analyses reveal that gene programs associated with lineage determination and oxidative stress are enriched in high engrafting cord blood, revealing potential molecular markers to be used as potency markers for cord blood unit selection regardless of length of cryopreservation. In summary, cord blood units cryopreserved for extended periods retain engrafting potential and can potentially be used for patient treatment.
KW - cryopreservation
KW - hematopoietic cell transplantation
KW - hematopoietic progenitor cells
KW - hematopoietic stem cells
KW - transcriptomics
KW - umbilical cord blood
UR - https://www.scopus.com/pages/publications/85177089256
U2 - 10.1016/j.xcrm.2023.101259
DO - 10.1016/j.xcrm.2023.101259
M3 - Article
C2 - 37913777
AN - SCOPUS:85177089256
SN - 2666-3791
VL - 4
JO - Cell Reports Medicine
JF - Cell Reports Medicine
IS - 11
M1 - 101259
ER -