Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer

Damien Soghoian, Vinodh Jayaraman, Michael Silane, Alejandro Berenstein, Thottala Jayaraman

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca2+) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca2+ release and Ca 2+-dependent signaling events in breast cancer.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalTumor Biology
Volume26
Issue number4
DOIs
StatePublished - 2005
Externally publishedYes

Keywords

  • Calcium
  • Cancer
  • Cell cycle
  • Phosphorylation
  • Signaling

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