Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer

Damien Soghoian; Vinodh Jayaraman; Michael Silane; Alejandro Berenstein; Thottala Jayaraman

doi:10.1159/000086954

Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer

Damien Soghoian, Vinodh Jayaraman, Michael Silane, Alejandro Berenstein, Thottala Jayaraman

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca²⁺) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca²⁺ release and Ca ²⁺-dependent signaling events in breast cancer.

Original language	English
Pages (from-to)	207-212
Number of pages	6
Journal	Tumor Biology
Volume	26
Issue number	4
DOIs	https://doi.org/10.1159/000086954
State	Published - 2005
Externally published	Yes

Keywords

Calcium
Cancer
Cell cycle
Phosphorylation
Signaling

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10.1159/000086954

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title = "Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer",

abstract = "The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca2+) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca2+ release and Ca 2+-dependent signaling events in breast cancer.",

keywords = "Calcium, Cancer, Cell cycle, Phosphorylation, Signaling",

author = "Damien Soghoian and Vinodh Jayaraman and Michael Silane and Alejandro Berenstein and Thottala Jayaraman",

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T1 - Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer

AU - Soghoian, Damien

AU - Jayaraman, Vinodh

AU - Silane, Michael

AU - Berenstein, Alejandro

AU - Jayaraman, Thottala

PY - 2005

Y1 - 2005

N2 - The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca2+) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca2+ release and Ca 2+-dependent signaling events in breast cancer.

AB - The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca2+) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca2+ release and Ca 2+-dependent signaling events in breast cancer.

KW - Calcium

KW - Cancer

KW - Cell cycle

KW - Phosphorylation

KW - Signaling

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U2 - 10.1159/000086954

DO - 10.1159/000086954

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EP - 212

JO - Tumor Biology

JF - Tumor Biology

SN - 1010-4283

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ER -

Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer

Abstract

Keywords

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