Injected Wnt RNA induces a complete body axis in Xenopus embryos

Sergei Sokol; Jan L. Christian; Randall T. Moon; Douglas A. Melton

doi:10.1016/0092-8674(91)90069-B

Injected Wnt RNA induces a complete body axis in Xenopus embryos

Sergei Sokol, Jan L. Christian, Randall T. Moon, Douglas A. Melton

Research output: Contribution to journal › Article › peer-review

454 Scopus citations

Abstract

Studies in Xenopus have shown that growth factors of the TGFβ and Wnt oncogene families can mimic aspects of dorsal axis formation. Here we directly compare the inductive properties of two Wnt proteins by injecting synthetic mRNA into developing embryos. The results show that Wnt-1 and Xwnt-8 can induce a new and complete dorsal axis and can rescue the development of axis-deficient, UV-irradiated embryos. In contrast, activin mRNA injection induces only a partial dorsal axis that lacks anterior structures. These studies demonstrate that the mechanism of Wnt-induced axis duplication results from the creation of an independent Spemann organizer. The relationship between the properties of the endogenous dorsal inducer and the effects of Wnts and activins is discussed.

Original language	English
Pages (from-to)	741-752
Number of pages	12
Journal	Cell
Volume	67
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(91)90069-B
State	Published - 15 Nov 1991
Externally published	Yes

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title = "Injected Wnt RNA induces a complete body axis in Xenopus embryos",

abstract = "Studies in Xenopus have shown that growth factors of the TGFβ and Wnt oncogene families can mimic aspects of dorsal axis formation. Here we directly compare the inductive properties of two Wnt proteins by injecting synthetic mRNA into developing embryos. The results show that Wnt-1 and Xwnt-8 can induce a new and complete dorsal axis and can rescue the development of axis-deficient, UV-irradiated embryos. In contrast, activin mRNA injection induces only a partial dorsal axis that lacks anterior structures. These studies demonstrate that the mechanism of Wnt-induced axis duplication results from the creation of an independent Spemann organizer. The relationship between the properties of the endogenous dorsal inducer and the effects of Wnts and activins is discussed.",

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AU - Sokol, Sergei

AU - Christian, Jan L.

AU - Moon, Randall T.

AU - Melton, Douglas A.

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Y1 - 1991/11/15

N2 - Studies in Xenopus have shown that growth factors of the TGFβ and Wnt oncogene families can mimic aspects of dorsal axis formation. Here we directly compare the inductive properties of two Wnt proteins by injecting synthetic mRNA into developing embryos. The results show that Wnt-1 and Xwnt-8 can induce a new and complete dorsal axis and can rescue the development of axis-deficient, UV-irradiated embryos. In contrast, activin mRNA injection induces only a partial dorsal axis that lacks anterior structures. These studies demonstrate that the mechanism of Wnt-induced axis duplication results from the creation of an independent Spemann organizer. The relationship between the properties of the endogenous dorsal inducer and the effects of Wnts and activins is discussed.

AB - Studies in Xenopus have shown that growth factors of the TGFβ and Wnt oncogene families can mimic aspects of dorsal axis formation. Here we directly compare the inductive properties of two Wnt proteins by injecting synthetic mRNA into developing embryos. The results show that Wnt-1 and Xwnt-8 can induce a new and complete dorsal axis and can rescue the development of axis-deficient, UV-irradiated embryos. In contrast, activin mRNA injection induces only a partial dorsal axis that lacks anterior structures. These studies demonstrate that the mechanism of Wnt-induced axis duplication results from the creation of an independent Spemann organizer. The relationship between the properties of the endogenous dorsal inducer and the effects of Wnts and activins is discussed.

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JO - Cell

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Injected Wnt RNA induces a complete body axis in Xenopus embryos

Abstract

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