Inhibitory effects of grape seed extract on lipases

Diego A. Moreno, Nebojsa Ilic, Alexander Poulev, Dawn L. Brasaemle, Susan K. Fried, Ilya Raskin

Research output: Contribution to journalArticlepeer-review

209 Scopus citations


OBJECTIVE: The aim of the present study was to assess the effects of grape seed extract (GSE) on the fat-metabolizing enzymes pancreatic lipase, lipoprotein lipase, and hormone-sensitive lipase in vitro and evaluate its potential application as a treatment for obesity. METHODS: Crushed grape seeds were extracted in ethanol, and the extract was assayed for the measurement of inhibitory effects on pancreatic lipase and lipoprotein lipase activities and on lipolysis of 3T3-L1 adipocytes. RESULTS: The GSE rich in bioactive phytochemicals showed inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase, thus suggesting that GSE might be useful as a treatment to limit dietary fat absorption and the accumulation of fat in adipose tissue. The observed reduction in intracellular lipolytic activity of cultured 3T3-L1 adipocytes may reduce the levels of circulating free fatty acids that have been linked to insulin resistance in obese patients. CONCLUSION: The GSE rich in compounds that inhibit lipases may provide a safe, natural, and cost-effective weight control treatment.

Original languageEnglish
Pages (from-to)876-879
Number of pages4
Issue number10
StatePublished - 1 Oct 2003


  • Natural products
  • Obesity
  • Plant extracts
  • Vitis vinifera


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