Inhibition of the ubiquitin-proteasome system affects influenza A virus infection at a postfusion step

Ivy Widjaja, Erik De Vries, Donna M. Tscherne, Adolfo García-Sastre, Peter J.M. Rottier, Cornelis A.M. De Haan

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

We have demonstrated that influenza A virus (IAV) RNA synthesis depends on the ubiquitin-proteasome system. IAV replication was reduced both by proteasome inhibitors and in E36ts20 cells, which contain the thermolabile ubiquitin-activating enzyme E1. While virus entry was not affected in E36ts20 cells, the proteasome inhibitor MG132 retained viral particles in the cytoplasm. Addition-removal experiments of MG132 in combination with bafilomycin A1, a well-established inhibitor of IAV entry and fusion, showed that MG132 affected IAV infection at a postfusion step. This was confirmed by the lack of inhibition of IAV entry by proteasome inhibitors in a virus-like particle fusion assay.

Original languageEnglish
Pages (from-to)9625-9631
Number of pages7
JournalJournal of Virology
Volume84
Issue number18
DOIs
StatePublished - Sep 2010

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