Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Rasaq Olajide Akinsola, Choon Weng Lee, Edmund Ui Hang Sim, Kumaran Narayanan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.

Original languageEnglish
Article number114088
JournalAnalytical Biochemistry
Volume616
DOIs
StatePublished - 1 Mar 2021
Externally publishedYes

Keywords

  • Bacteria DNA delivery
  • Endocytosed
  • Endosome
  • Lysosome
  • Proton pump inhibitor
  • V-ATPase

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