Inhibition of cell mediated cytolysis of trinitrophenyl derivatized target cells by alloantisera directed to the products of the K and D loci of the H 2 complex

S. J. Burakoff, R. N. Germain, M. E. Dorf, B. Benacerraf

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56 Scopus citations

Abstract

The role of the products of the K and D loci of the H 2 complex as target antigens for cytotoxic T cells generated to modified syngeneic cells has been investigated. Spleen cells, when cocultured with trinitrophenyl derivatized syngeneic spleen cells, generate cytolytic T cells that lyse most effectively tumor or spleen targets that are trinitrophenyl derivatized and H 2 identical to the sensitizing cell. Cytolysis is inhibited by alloantisera to the K and/or D specificities of the target cells and by anti dinitrophenyl antisera. Cytolytic cells generated in this manner are also able to lyse, though less efficiently, trinitrophenyl derivatized tumor and spleen targets that do not share products of the K and D loci with the sensitizing cell, and this cytolysis is also blocked by alloantisera directed to H 2 serological specificities of such targets.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

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