Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor

Shengqi Li; Yongyan Tan; Peng Wang; Jinqing Kan

doi:10.1016/j.snb.2009.08.038

Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor

Shengqi Li, Yongyan Tan, Peng Wang, Jinqing Kan

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A polyaniline-polyphenol oxidase (PANI-PPO) biosensor for detecting benzoic acid is reported. The biosensor is based on the inhibition of benzoic acid on the biocatalytic activity of the polyphenol oxidase (PPO). The Michaelis-Menten constant (k^′_m) and maximum response current (I_max) in both the absence and presence of benzoic acid are also evaluated. The kinetic analyses show that the inhibition of benzoic acid on the PPO activity is reversible and competitive with an inhibition constant of 28.7 μmol dm^-3. A limit of detection of 0.3 μmol dm^-3 benzoic acid is obtained. The I_0.5 value is estimated to be 35 μmol dm^-3. The biosensor has good sensitivity and high stability. So it can be used as a new approach for the detecting benzoic acid.

Original language	English
Pages (from-to)	18-22
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	144
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2009.08.038
State	Published - 29 Jan 2010
Externally published	Yes

Keywords

Benzoic acid
Biosensor
Inhibition
Polyaniline
Polyphenol oxidase

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10.1016/j.snb.2009.08.038

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title = "Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor",

abstract = "A polyaniline-polyphenol oxidase (PANI-PPO) biosensor for detecting benzoic acid is reported. The biosensor is based on the inhibition of benzoic acid on the biocatalytic activity of the polyphenol oxidase (PPO). The Michaelis-Menten constant (k′m) and maximum response current (Imax) in both the absence and presence of benzoic acid are also evaluated. The kinetic analyses show that the inhibition of benzoic acid on the PPO activity is reversible and competitive with an inhibition constant of 28.7 μmol dm-3. A limit of detection of 0.3 μmol dm-3 benzoic acid is obtained. The I0.5 value is estimated to be 35 μmol dm-3. The biosensor has good sensitivity and high stability. So it can be used as a new approach for the detecting benzoic acid.",

keywords = "Benzoic acid, Biosensor, Inhibition, Polyaniline, Polyphenol oxidase",

author = "Shengqi Li and Yongyan Tan and Peng Wang and Jinqing Kan",

note = "Funding Information: This project was supported by National Science Foundation of China (No. 20873119 ). ",

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T1 - Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor

AU - Li, Shengqi

AU - Tan, Yongyan

AU - Wang, Peng

AU - Kan, Jinqing

N1 - Funding Information: This project was supported by National Science Foundation of China (No. 20873119 ).

PY - 2010/1/29

Y1 - 2010/1/29

N2 - A polyaniline-polyphenol oxidase (PANI-PPO) biosensor for detecting benzoic acid is reported. The biosensor is based on the inhibition of benzoic acid on the biocatalytic activity of the polyphenol oxidase (PPO). The Michaelis-Menten constant (k′m) and maximum response current (Imax) in both the absence and presence of benzoic acid are also evaluated. The kinetic analyses show that the inhibition of benzoic acid on the PPO activity is reversible and competitive with an inhibition constant of 28.7 μmol dm-3. A limit of detection of 0.3 μmol dm-3 benzoic acid is obtained. The I0.5 value is estimated to be 35 μmol dm-3. The biosensor has good sensitivity and high stability. So it can be used as a new approach for the detecting benzoic acid.

AB - A polyaniline-polyphenol oxidase (PANI-PPO) biosensor for detecting benzoic acid is reported. The biosensor is based on the inhibition of benzoic acid on the biocatalytic activity of the polyphenol oxidase (PPO). The Michaelis-Menten constant (k′m) and maximum response current (Imax) in both the absence and presence of benzoic acid are also evaluated. The kinetic analyses show that the inhibition of benzoic acid on the PPO activity is reversible and competitive with an inhibition constant of 28.7 μmol dm-3. A limit of detection of 0.3 μmol dm-3 benzoic acid is obtained. The I0.5 value is estimated to be 35 μmol dm-3. The biosensor has good sensitivity and high stability. So it can be used as a new approach for the detecting benzoic acid.

KW - Benzoic acid

KW - Biosensor

KW - Inhibition

KW - Polyaniline

KW - Polyphenol oxidase

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DO - 10.1016/j.snb.2009.08.038

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SN - 0925-4005

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 1

ER -

Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor

Abstract

Keywords

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