Infrequent MDM2 gene amplification and absence of gross WAF1 gene alterations in nasopharyngeal carcinoma

Wang Qian, Li Fu Hu, Fu Chen, Yisong Wang, K. P. Magnusson, E. Kashuba, G. Klein, K. G. Wiman

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

We have investigated the possible involvement of MDM2 and WAF1 gene alterations in the development of nasopharyngeal carcinoma (NPC). MDM2 and WAF1 were analysed in 46 primary NPCs by Southern blot analysis. Forty-five tumours showed a normal EcoRI hybridisation pattern and hybridisation intensity with a human MDM2 cDNA probe. One tumour had more intense normal size MDM2 hybridising bands. Densitometric scanning revealed a 10-12-fold MDM2 gene amplification, as compared with human placenta DNA. All 46 tumours showed normal size WAF1 EcoRI bands that hybridised with normal intensity. This is the first demonstration of MDM2 gene amplification in NPC. Nonetheless, our analysis indicates that gross structural alterations of the MDM2 and WAF1 genes are infrequent events in the genesis of NPC.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
JournalEuropean Journal of Cancer. Part B: Oral Oncology
Volume31
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • MDM2 amplification
  • NPC
  • WAF1

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