TY - JOUR
T1 - Influenza A virus haemagglutinin polymorphism
T2 - Pleiotropic antigenic variants of A/Shanghai/11/87 (H3N2) virus selected as high yield reassortants
AU - Kilbourne, E. D.
AU - Johansson, B. E.
AU - Moran, T.
AU - Wu, S.
AU - Pokorny, B. A.
AU - Xu, X.
AU - Cox, N.
PY - 1993
Y1 - 1993
N2 - Genetic reassortment of the A/Shanghai/11/87 (H3N2) variant of influenza A virus with A/PR8/34 (H1N1) virus [the standard donor of high yield (hy) genes for influenza vaccine viruses] resulted in the isolation of two reassortants with differing H3 haemagglutinin (HA) phenotypes, X-99 and X-99a. The two HA phenotypes were derived from individual subpopulations of the H3N2 wild-type virus during the reassortment event. The HA mutants and their respectively derived reassortants (identical in RNA genotype) differed in antigenicity, replication characteristics, yield in chick embryos and haemagglutinin gene sequence. Despite antigenic differences in reactions to polyclonal rabbit antisera of 60%, both X-99 and X-99a, the hy reassortants, were equally immunogenic and protective in BALB/c mice to challenge by parental wild-type virus. Differences in HA phenotype were related to a Ser to Ile change at amino acid position 186. These findings emphasize the polymorphism of influenza virus strains as well as the need for caution in selection of vaccine strains from among antigenically distinct viral subpopulations.
AB - Genetic reassortment of the A/Shanghai/11/87 (H3N2) variant of influenza A virus with A/PR8/34 (H1N1) virus [the standard donor of high yield (hy) genes for influenza vaccine viruses] resulted in the isolation of two reassortants with differing H3 haemagglutinin (HA) phenotypes, X-99 and X-99a. The two HA phenotypes were derived from individual subpopulations of the H3N2 wild-type virus during the reassortment event. The HA mutants and their respectively derived reassortants (identical in RNA genotype) differed in antigenicity, replication characteristics, yield in chick embryos and haemagglutinin gene sequence. Despite antigenic differences in reactions to polyclonal rabbit antisera of 60%, both X-99 and X-99a, the hy reassortants, were equally immunogenic and protective in BALB/c mice to challenge by parental wild-type virus. Differences in HA phenotype were related to a Ser to Ile change at amino acid position 186. These findings emphasize the polymorphism of influenza virus strains as well as the need for caution in selection of vaccine strains from among antigenically distinct viral subpopulations.
UR - http://www.scopus.com/inward/record.url?scp=0027282159&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-74-7-1311
DO - 10.1099/0022-1317-74-7-1311
M3 - Article
C2 - 8336120
AN - SCOPUS:0027282159
SN - 0022-1317
VL - 74
SP - 1311
EP - 1316
JO - Journal of General Virology
JF - Journal of General Virology
IS - 7
ER -