Influence of Early Life Factors, including breast milk Composition, on the Microbiome of Infants Born to Mothers with and without Inflammatory Bowel Disease

J. Sabino, L. Tarassishin, C. Eisele, K. Hawkins, A. Barré, N. Nair, A. Rendon, A. Debebe, M. Picker, M. Agrawal, J. Stone, J. George, Peter Legnani, Elana Maser, Ching Lynn Chen, Anne Thjømøe, Einar Mørk, M. Dubinsky, J. Hu, J. F. ColombelI. Peter, Joana Torres

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background and Aims: Herein we analysed the influence of early life factors, including breast milk composition, on the development of the intestinal microbiota of infants born to mothers with and without IBD. Methods: The MECONIUM [Exploring MEChanisms Of disease traNsmission In Utero through the Microbiome] study is a prospective cohort study consisting of pregnant women with or without IBD and their infants. Longitudinal stool samples were collected from babies and analysed using 16s rRNA sequencing and faecal calprotectin. Breast milk proteomics was profiled using Olink inflammation panel. Results: We analysed gut microbiota of 1034 faecal samples from 294 infants [80 born to mothers with and 214 to mothers without IBD]. Alpha diversity was driven by maternal IBD status and time point. The major influencers of the overall composition of the microbiota were mode of delivery, feeding, and maternal IBD status. Specific taxa were associated with these exposures, and maternal IBD was associated with a reduction in Bifidobacterium. In 312 breast milk samples [91 from mothers with IBD], mothers with IBD displayed lower abundance of proteins involved in immune regulation, such as thymic stromal lymphopoietin, interleukin-12 subunit beta, tumour necrosis factor-beta, and C-C motif chemokine 20, as compared with control mothers [adjusted p=0.0016, 0.049, 0.049, and 0.049, respectively], with negative correlations with babýs calprotectin, and microbiome at different time points. Conclusion: Maternal IBD diagnosis influences microbiota in their offspring during early life. The proteomic profile of breast milk of women with IBD differs from that of women without IBD, with distinct time-dependent associations with baby's gut microbiome and feacal calprotectin.

Original languageEnglish
Pages (from-to)1723-1732
Number of pages10
JournalJournal of Crohn's and Colitis
Volume17
Issue number11
DOIs
StatePublished - 1 Nov 2023

Keywords

  • Early life
  • IBD
  • breast milk
  • microbiota

Fingerprint

Dive into the research topics of 'Influence of Early Life Factors, including breast milk Composition, on the Microbiome of Infants Born to Mothers with and without Inflammatory Bowel Disease'. Together they form a unique fingerprint.

Cite this