Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P2O5-60SiO2-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses

Rupinderjeet Bains; Piyush Sharma; Rameez Ahmad Mir; Suninder Jeet; Gurbinder Kaur; Om Prakash Pandey

doi:10.1002/jbm.a.36415

Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses

Rupinderjeet Bains
, Piyush Sharma
, Rameez Ahmad Mir
, Suninder Jeet
, Gurbinder Kaur
, Om Prakash Pandey

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

In the present study, novel mesoporous bioactive glasses (MBGs) (15-x)CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5, varying in steps of 2) are synthesized using the sol-gel technique. The structural phases of the glasses/glass ceramics were studied by XRD. The pH variation and simulated body fluids (SBF) studies demonstrated the in-vitro bioactivity of all the MBGs. MBGs possess surface area variation between 98.22 and 442.41 cm²/g. The pore size of MBGs lies in the range of 5.8–8.8 nm. The cytotoxicity assays were conducted for MG63 human osteosarcoma cell line depicting non-toxic behavior of all MBGs at 7.8125 μg/ml. In addition to this, the effect of the magnesium on the gene expression was also investigated using reverse transcription–polymerase chain reaction (RT-qPCR). The MBGs were loaded with the antibacterial (vancomycin/amoxicillin), anticancerous (doxorubicin), and analgesic (Iburofen) drugs. Ibuprofen and amoxicillin drugs were almost fully loaded in all the MBGs, whereas doxorubicin and vancomycin drugs illustrated variation in loading with decreasing copper content.

Original language	English
Pages (from-to)	2116-2130
Number of pages	15
Journal	Journal of Biomedical Materials Research - Part A
Volume	106
Issue number	8
DOIs	https://doi.org/10.1002/jbm.a.36415
State	Published - Aug 2018
Externally published	Yes

Keywords

Ibuprofen
MG63 osteosarcoma cell line
RT-qPCR
amoxicillin
doxorubicin
drug loading
gene expresssion
mesoporous bioactive glasses (MBGs)
vancomycin

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Bains, R., Sharma, P., Mir, R. A., Jeet, S., Kaur, G., & Pandey, O. P. (2018). Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses. Journal of Biomedical Materials Research - Part A, 106(8), 2116-2130. https://doi.org/10.1002/jbm.a.36415

Bains, Rupinderjeet ; Sharma, Piyush ; Mir, Rameez Ahmad et al. / Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses. In: Journal of Biomedical Materials Research - Part A. 2018 ; Vol. 106, No. 8. pp. 2116-2130.

@article{1166121d3d1f427a9249f6824c4b0fbd,

title = "Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P2O5-60SiO2-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses",

abstract = "In the present study, novel mesoporous bioactive glasses (MBGs) (15-x)CuO-xMgO-10P2O5-60SiO2-10CaO-5ZnO (2.5 ≤ x ≤ 12.5, varying in steps of 2) are synthesized using the sol-gel technique. The structural phases of the glasses/glass ceramics were studied by XRD. The pH variation and simulated body fluids (SBF) studies demonstrated the in-vitro bioactivity of all the MBGs. MBGs possess surface area variation between 98.22 and 442.41 cm2/g. The pore size of MBGs lies in the range of 5.8{\textendash}8.8 nm. The cytotoxicity assays were conducted for MG63 human osteosarcoma cell line depicting non-toxic behavior of all MBGs at 7.8125 μg/ml. In addition to this, the effect of the magnesium on the gene expression was also investigated using reverse transcription{\textendash}polymerase chain reaction (RT-qPCR). The MBGs were loaded with the antibacterial (vancomycin/amoxicillin), anticancerous (doxorubicin), and analgesic (Iburofen) drugs. Ibuprofen and amoxicillin drugs were almost fully loaded in all the MBGs, whereas doxorubicin and vancomycin drugs illustrated variation in loading with decreasing copper content.",

keywords = "Ibuprofen, MG63 osteosarcoma cell line, RT-qPCR, amoxicillin, doxorubicin, drug loading, gene expresssion, mesoporous bioactive glasses (MBGs), vancomycin",

author = "Rupinderjeet Bains and Piyush Sharma and Mir, \{Rameez Ahmad\} and Suninder Jeet and Gurbinder Kaur and Pandey, \{Om Prakash\}",

note = "Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2018",

month = aug,

doi = "10.1002/jbm.a.36415",

language = "English",

volume = "106",

pages = "2116--2130",

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Bains, R, Sharma, P, Mir, RA, Jeet, S, Kaur, G & Pandey, OP 2018, 'Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses', Journal of Biomedical Materials Research - Part A, vol. 106, no. 8, pp. 2116-2130. https://doi.org/10.1002/jbm.a.36415

Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses. / Bains, Rupinderjeet; Sharma, Piyush; Mir, Rameez Ahmad et al.
In: Journal of Biomedical Materials Research - Part A, Vol. 106, No. 8, 08.2018, p. 2116-2130.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P2O5-60SiO2-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses

AU - Bains, Rupinderjeet

AU - Sharma, Piyush

AU - Mir, Rameez Ahmad

AU - Jeet, Suninder

AU - Kaur, Gurbinder

AU - Pandey, Om Prakash

PY - 2018/8

Y1 - 2018/8

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KW - MG63 osteosarcoma cell line

KW - RT-qPCR

KW - amoxicillin

KW - doxorubicin

KW - drug loading

KW - gene expresssion

KW - mesoporous bioactive glasses (MBGs)

KW - vancomycin

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Bains R, Sharma P, Mir RA, Jeet S, Kaur G, Pandey OP. Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15-x) CuO-xMgO-10P₂O₅-60SiO₂-10CaO-5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses. Journal of Biomedical Materials Research - Part A. 2018 Aug;106(8):2116-2130. doi: 10.1002/jbm.a.36415