TY - JOUR
T1 - Infection of the human T-cell-derived leukemia line Molt-4 by Epstein-Barr virus (EBV)
T2 - Induction of EBV-determined antigens and virus reproduction
AU - Shapiro, Ilya M.
AU - Volsky, David J.
AU - Saemundsen, Ari K.
AU - Anisimova, Emma
AU - Klein, George
N1 - Funding Information:
This work is supported in part by NC1 2ROl CA 14054-07Al and by the Swedish Cancer Society. D.J.V. was the recipient of a long-term fellowship from the European Molecular Biology Organization.
PY - 1982/7/15
Y1 - 1982/7/15
N2 - We have reported previously that human T-cell-derived Molt-4 cells become susceptible to Epstein-Barr virus (EBV) infection after implantation of functional EBV receptors into the plasma membranes of Molt-4 cells (Volsky et al., 1980). In the present work, we expand this finding by analyzing the following: (i) Virus adsorption vs viral penetration-using [3H]thymidine-labeled EBV, we demonstrate that the virus could adsorb to both the untreated and the receptor-implanted Molt-4 cells. However, only the altered cells became susceptible to EBV penetration followed by the viral antigen synthesis; (ii) EBV substrain specificity of infection-EBV P3HR-1 virus induced the nuclear (EBNA), early (EA), and virus capsid (VCA) antigens, whereas EBV B-95-8 virus induced only EBNA; (iii) virus reproduction-in situ hybridization was used to demonstrate the EBV-DNA synthesis in the P3HR-1 virus-infected cells. In addition, immature herpes-like particles were observed in electron micrographs of infected cells. It is concluded that EBV infection of human T-cell-derived Molt-4 cells may lead to a full viral-lytic cycle in a portion of infected cells. The results suggest, however, that the primary EBV productive infection may not necessarily involve any immunofluorescence-detectable EBNA synthesis.
AB - We have reported previously that human T-cell-derived Molt-4 cells become susceptible to Epstein-Barr virus (EBV) infection after implantation of functional EBV receptors into the plasma membranes of Molt-4 cells (Volsky et al., 1980). In the present work, we expand this finding by analyzing the following: (i) Virus adsorption vs viral penetration-using [3H]thymidine-labeled EBV, we demonstrate that the virus could adsorb to both the untreated and the receptor-implanted Molt-4 cells. However, only the altered cells became susceptible to EBV penetration followed by the viral antigen synthesis; (ii) EBV substrain specificity of infection-EBV P3HR-1 virus induced the nuclear (EBNA), early (EA), and virus capsid (VCA) antigens, whereas EBV B-95-8 virus induced only EBNA; (iii) virus reproduction-in situ hybridization was used to demonstrate the EBV-DNA synthesis in the P3HR-1 virus-infected cells. In addition, immature herpes-like particles were observed in electron micrographs of infected cells. It is concluded that EBV infection of human T-cell-derived Molt-4 cells may lead to a full viral-lytic cycle in a portion of infected cells. The results suggest, however, that the primary EBV productive infection may not necessarily involve any immunofluorescence-detectable EBNA synthesis.
UR - http://www.scopus.com/inward/record.url?scp=0020028795&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(82)90015-0
DO - 10.1016/0042-6822(82)90015-0
M3 - Article
C2 - 6285596
AN - SCOPUS:0020028795
SN - 0042-6822
VL - 120
SP - 171
EP - 181
JO - Virology
JF - Virology
IS - 1
ER -