Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate

Janos Luka; Bengt Kallin; George Klein

doi:10.1016/0042-6822(79)90455-0

Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate

Janos Luka, Bengt Kallin, George Klein

Research output: Contribution to journal › Article › peer-review

334 Scopus citations

Abstract

n-Butyrate was found to increase the number of virus producer cells to a dramatic extent in the Epstein-Barr virus (EBV)-carrying P3HR-1 and B95-8 lines. Induction was also seen in the nonproducer Raji and the low producer Daudi lines, but at a mucch lower level. The virus containing supernatant of the butyrate treated P3HR-1 cells induced preferentially EBNA in EBV-negative Ramos target cells, whereas the spontaneously produced virus induced predominantly EA in Raji indicator cells. This suggests a possible difference in the biological properties of the butyrate induced vs the prototype virus. In addition to providing a convenient method to obtain a high yield of viral-DNA and virus antigen-producing cells in the severely restricted EBV system, the findings raise interesting questions on the mechanism of EBV induction, and its possible relationship to the known differentiation inducing ability of n-butyrate.

Original language	English
Pages (from-to)	228-231
Number of pages	4
Journal	Virology
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(79)90455-0
State	Published - 15 Apr 1979
Externally published	Yes

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title = "Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate",

abstract = "n-Butyrate was found to increase the number of virus producer cells to a dramatic extent in the Epstein-Barr virus (EBV)-carrying P3HR-1 and B95-8 lines. Induction was also seen in the nonproducer Raji and the low producer Daudi lines, but at a mucch lower level. The virus containing supernatant of the butyrate treated P3HR-1 cells induced preferentially EBNA in EBV-negative Ramos target cells, whereas the spontaneously produced virus induced predominantly EA in Raji indicator cells. This suggests a possible difference in the biological properties of the butyrate induced vs the prototype virus. In addition to providing a convenient method to obtain a high yield of viral-DNA and virus antigen-producing cells in the severely restricted EBV system, the findings raise interesting questions on the mechanism of EBV induction, and its possible relationship to the known differentiation inducing ability of n-butyrate.",

author = "Janos Luka and Bengt Kallin and George Klein",

note = "Funding Information: 1 This project has been funded in part with Federal funds from the Department of Health, Education and Welfare under Contract NO1 CP 33316 and by the Swedish Cancer Society. * To whom reprint requests should be addressed.",

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doi = "10.1016/0042-6822(79)90455-0",

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T1 - Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate

AU - Luka, Janos

AU - Kallin, Bengt

AU - Klein, George

N1 - Funding Information: 1 This project has been funded in part with Federal funds from the Department of Health, Education and Welfare under Contract NO1 CP 33316 and by the Swedish Cancer Society. * To whom reprint requests should be addressed.

PY - 1979/4/15

Y1 - 1979/4/15

N2 - n-Butyrate was found to increase the number of virus producer cells to a dramatic extent in the Epstein-Barr virus (EBV)-carrying P3HR-1 and B95-8 lines. Induction was also seen in the nonproducer Raji and the low producer Daudi lines, but at a mucch lower level. The virus containing supernatant of the butyrate treated P3HR-1 cells induced preferentially EBNA in EBV-negative Ramos target cells, whereas the spontaneously produced virus induced predominantly EA in Raji indicator cells. This suggests a possible difference in the biological properties of the butyrate induced vs the prototype virus. In addition to providing a convenient method to obtain a high yield of viral-DNA and virus antigen-producing cells in the severely restricted EBV system, the findings raise interesting questions on the mechanism of EBV induction, and its possible relationship to the known differentiation inducing ability of n-butyrate.

AB - n-Butyrate was found to increase the number of virus producer cells to a dramatic extent in the Epstein-Barr virus (EBV)-carrying P3HR-1 and B95-8 lines. Induction was also seen in the nonproducer Raji and the low producer Daudi lines, but at a mucch lower level. The virus containing supernatant of the butyrate treated P3HR-1 cells induced preferentially EBNA in EBV-negative Ramos target cells, whereas the spontaneously produced virus induced predominantly EA in Raji indicator cells. This suggests a possible difference in the biological properties of the butyrate induced vs the prototype virus. In addition to providing a convenient method to obtain a high yield of viral-DNA and virus antigen-producing cells in the severely restricted EBV system, the findings raise interesting questions on the mechanism of EBV induction, and its possible relationship to the known differentiation inducing ability of n-butyrate.

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JO - Virology

JF - Virology

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ER -

Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate

Abstract

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