Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Ming Qiao; Mundrigi Ashok; Kristen A. Bernard; Gustavo Palacios; Z. Hong Zhou; W. Ian Lipkin; T. Jake Liang

doi:10.1086/425933

Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Ming Qiao
, Mundrigi Ashok
, Kristen A. Bernard
, Gustavo Palacios
, Z. Hong Zhou
, W. Ian Lipkin
, T. Jake Liang

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

Original language	English
Pages (from-to)	2104-2108
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	190
Issue number	12
DOIs	https://doi.org/10.1086/425933
State	Published - 15 Dec 2004
Externally published	Yes

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10.1086/425933

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@article{ca6655e38c7540d58277ff41f02005d4,

title = "Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells",

abstract = "No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.",

author = "Ming Qiao and Mundrigi Ashok and Bernard, \{Kristen A.\} and Gustavo Palacios and Zhou, \{Z. Hong\} and Lipkin, \{W. Ian\} and Liang, \{T. Jake\}",

note = "Funding Information: Financial support: National Institutes of Health (grant U54AI57158-Lipkin to W.I.L., A.M., and K.B. and grant AI51292 to W.I.L., A.M., and G.P.); Ellison Medical Foundation (to W.I.L.); New York State Department of Health (support to K.B.).",

year = "2004",

month = dec,

day = "15",

doi = "10.1086/425933",

language = "English",

volume = "190",

pages = "2104--2108",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "12",

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TY - JOUR

T1 - Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

AU - Qiao, Ming

AU - Ashok, Mundrigi

AU - Bernard, Kristen A.

AU - Palacios, Gustavo

AU - Zhou, Z. Hong

AU - Lipkin, W. Ian

AU - Liang, T. Jake

N1 - Funding Information: Financial support: National Institutes of Health (grant U54AI57158-Lipkin to W.I.L., A.M., and K.B. and grant AI51292 to W.I.L., A.M., and G.P.); Ellison Medical Foundation (to W.I.L.); New York State Department of Health (support to K.B.).

PY - 2004/12/15

Y1 - 2004/12/15

N2 - No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

AB - No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

UR - https://www.scopus.com/pages/publications/10444219598

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DO - 10.1086/425933

M3 - Article

C2 - 15551208

AN - SCOPUS:10444219598

SN - 0022-1899

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SP - 2104

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JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 12

ER -

Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Abstract

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