Induction of liver microsomal monooxygenases in experimental cholestasis

N. E. Zakharova, V. A. Mordvinov, I. B. Tsyrlov, V. V. Lyakhovich

Research output: Contribution to journalArticlepeer-review

Abstract

The intensity of the inducing action of phenobarbital on microsomal monooxygenase depending on the number of binding sites for this inducer in the active center of cytochrome P-450 was investigated. Cholestasis accompanied by the accumulation of hydroxylated metabolites of cholesterol, possessing detergent properties and disintegrating the substrate area for phenobarbital in the P-450 molecule, was chosen as the model. The ability of phenobarbital to induce under conditions excluding the stage of primary binding and metabolism of monooxygenase was demonstrated; this points to activation of the synthesis of the specific protein by the whole molecule of the inducer and not by its primary hydroxylation products in the microsomes.

Original languageEnglish
Pages (from-to)509-512
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume81
Issue number4
DOIs
StatePublished - Apr 1976

Keywords

  • cholestasis
  • induction by phenobarbital
  • microsomal monooxygenase

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