TY - JOUR
T1 - Induction and biological characterization of the Epstein-Barr virus (EBV) carried by the Jijoye lymphoma line
AU - Ragona, Giuseppe
AU - Ernberg, Ingemar
AU - Klein, George
N1 - Funding Information:
This research was supported( in part) by Contract No. NO1 CP 33316 from the Division of Cancer Cause and Prevention, National Cancer Institute, the Swedish Cancer Society, and King Gustaf V Jubilee Fund.
Funding Information:
1 G.R. is a recipient of a fellowship from the Fonda-zione “Instituto Pasteur-Fondaaione Cenci-Bolognetti,” Rome, Italy. * To whom reprint requests should be addressed.
PY - 1980/3
Y1 - 1980/3
N2 - In contrast to all other EBV isolates tested, the P3HR-1 line, a clonal derivative of the Burkitt lymphoma line Jijoye, releases nontransforming, cytopathic virus, which induces an abortive cycle in superinfected, EBV-receptor-positive lymphoid cell lines. We have examined the question whether P3HR-1 virus is a unique mutant or if the original, Jijoye-associated virus had already similar biological characteristics,. Jijoye cells were induced with sodium butyrate and with anti-IgM, respectively. Transforming virus was recovered, capable of inducing EBNA in cord blood cells. Both human cord blood and marmoset lymphocytes were immortalized by Jijoye virus. Both derived lines showed a typical lymphoblastoid (LCL) phenotype, including agarose clonability. It is concluded that P3HR-1 virus is a mutant of the original transforming Jijoye virus. Moreover, the results suggest that the phenotypic properties of the transformed lines are determined at the cellular, not the viral genome level.
AB - In contrast to all other EBV isolates tested, the P3HR-1 line, a clonal derivative of the Burkitt lymphoma line Jijoye, releases nontransforming, cytopathic virus, which induces an abortive cycle in superinfected, EBV-receptor-positive lymphoid cell lines. We have examined the question whether P3HR-1 virus is a unique mutant or if the original, Jijoye-associated virus had already similar biological characteristics,. Jijoye cells were induced with sodium butyrate and with anti-IgM, respectively. Transforming virus was recovered, capable of inducing EBNA in cord blood cells. Both human cord blood and marmoset lymphocytes were immortalized by Jijoye virus. Both derived lines showed a typical lymphoblastoid (LCL) phenotype, including agarose clonability. It is concluded that P3HR-1 virus is a mutant of the original transforming Jijoye virus. Moreover, the results suggest that the phenotypic properties of the transformed lines are determined at the cellular, not the viral genome level.
UR - http://www.scopus.com/inward/record.url?scp=0018849318&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(80)90473-0
DO - 10.1016/0042-6822(80)90473-0
M3 - Article
C2 - 6244705
AN - SCOPUS:0018849318
SN - 0042-6822
VL - 101
SP - 553
EP - 557
JO - Virology
JF - Virology
IS - 2
ER -