TY - JOUR
T1 - Induced reactive oxygen species improve enzyme production from Aspergillus niger cultivation
AU - Sahoo, Susmita
AU - Krishnamurthy Rao, K.
AU - Suraishkumar, G. K.
PY - 2003/5
Y1 - 2003/5
N2 - Intracellular reactive oxygen species (iROS) induction by HOCl was used as a novel strategy to improve enzyme productivities in Aspergillus niger growing in a bioreactor. With induced iROS, the specific intracellular activities of α-amylase, protease, catalase, and glucose oxidase were increased by about 170%, 250%, 320%, and 260%, respectively. The optimum specific iROS level for achieving maximum cell concentration and enzyme production was about 15 mmol g cell-1. The type of iROS inducing the enzyme production was identified to be a derivative of the superoxide radical.
AB - Intracellular reactive oxygen species (iROS) induction by HOCl was used as a novel strategy to improve enzyme productivities in Aspergillus niger growing in a bioreactor. With induced iROS, the specific intracellular activities of α-amylase, protease, catalase, and glucose oxidase were increased by about 170%, 250%, 320%, and 260%, respectively. The optimum specific iROS level for achieving maximum cell concentration and enzyme production was about 15 mmol g cell-1. The type of iROS inducing the enzyme production was identified to be a derivative of the superoxide radical.
KW - Aspergillus niger
KW - Enzyme productivity
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=0037904749&partnerID=8YFLogxK
U2 - 10.1023/A:1023508609623
DO - 10.1023/A:1023508609623
M3 - Article
C2 - 12882014
AN - SCOPUS:0037904749
SN - 0141-5492
VL - 25
SP - 821
EP - 825
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 10
ER -