TY - JOUR
T1 - Induced Pluripotent Stem Cells
T2 - Fundamentals and Applications of the Reprogramming Process and its Ramifications on Regenerative Medicine
AU - Walia, Bhavita
AU - Satija, Neeraj
AU - Tripathi, Rajendra Prashad
AU - Gangenahalli, Gurudutta U.
PY - 2012/3
Y1 - 2012/3
N2 - Objective: To provide a comprehensive source of information about the reprogramming process and induced pluripotency. Background: The ability of stem cells to renew their own population and to differentiate into specialized cell types has always attracted researchers looking to exploit this potential for cellular replacement therapies, pharmaceutical testing and studying developmental pathways. While adult stem cell therapy has already been brought to the clinic, embryonic stem cell research has been beset with legal and ethical impediments. Focus: The conversion of human somatic cells to human induced pluripotent stem cells (hiPSCs), which are equivalent to human embryonic stem cells (hESCs), provides a system to sidestep these barriers and expedite pluripotent stem cell research for the aforementioned purposes. However, being a very recent discovery, iPSCs have yet to overcome many other obstacles and criticism to be proven safe and feasible for clinical use. Methodology: This review introduces iPSC, the various methods that have been used to generate them and their pros and cons. It also covers in detail the pluripotency factors responsible for iPSC generation as well as the signaling pathways, epigenetic modifications and miRNA regulation implicated in the reprogramming process. The known molecular crosstalk between these reprogramming regulators is also illuminated. We will also mention the molecular compounds which have been shown to either replace one or more genetic factors or improve overall efficiency and kinetics of iPSC induction. Conclusion: To conclude, we will briefly discuss the current problems that hinder bench to bedside translation of iPSC research as well as the possible steps that can bring iPSC therapy and other potential applications closer to fruition.
AB - Objective: To provide a comprehensive source of information about the reprogramming process and induced pluripotency. Background: The ability of stem cells to renew their own population and to differentiate into specialized cell types has always attracted researchers looking to exploit this potential for cellular replacement therapies, pharmaceutical testing and studying developmental pathways. While adult stem cell therapy has already been brought to the clinic, embryonic stem cell research has been beset with legal and ethical impediments. Focus: The conversion of human somatic cells to human induced pluripotent stem cells (hiPSCs), which are equivalent to human embryonic stem cells (hESCs), provides a system to sidestep these barriers and expedite pluripotent stem cell research for the aforementioned purposes. However, being a very recent discovery, iPSCs have yet to overcome many other obstacles and criticism to be proven safe and feasible for clinical use. Methodology: This review introduces iPSC, the various methods that have been used to generate them and their pros and cons. It also covers in detail the pluripotency factors responsible for iPSC generation as well as the signaling pathways, epigenetic modifications and miRNA regulation implicated in the reprogramming process. The known molecular crosstalk between these reprogramming regulators is also illuminated. We will also mention the molecular compounds which have been shown to either replace one or more genetic factors or improve overall efficiency and kinetics of iPSC induction. Conclusion: To conclude, we will briefly discuss the current problems that hinder bench to bedside translation of iPSC research as well as the possible steps that can bring iPSC therapy and other potential applications closer to fruition.
KW - Cell therapy
KW - Embryonic stem cells
KW - Induced pluripotent stem cells
KW - Nanog
KW - Oct4
KW - Regenerative medicine
KW - Reprogramming factors
KW - Sox2
KW - iPSCs
UR - http://www.scopus.com/inward/record.url?scp=84857794026&partnerID=8YFLogxK
U2 - 10.1007/s12015-011-9279-x
DO - 10.1007/s12015-011-9279-x
M3 - Review article
C2 - 21671061
AN - SCOPUS:84857794026
SN - 1550-8943
VL - 8
SP - 100
EP - 115
JO - Stem Cell Reviews and Reports
JF - Stem Cell Reviews and Reports
IS - 1
ER -