Independent expression of human α or β platelet-derived growth factor receptor cDNAs in a naive hematopoietic cell leads to functional coupling with mitogenic ahd chemotactic signaling pathways

T. Matsui, J. H. Pierce, T. P. Fleming, J. S. Greenberger, W. J. LaRochelle, M. Ruggiero, S. A. Aaronson

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141 Scopus citations

Abstract

Distinct genes encode α and β platelet-derived growth factor (PDGF) receptors that differ in their abilities to be triggered by three dimeric forms of the PDGF molecule. We show that PDGF-receptor mitogenic function can be reconstituted in a naive hematopoietic cell line by introduction of expression vectors for either α or β PDGF receptor cDNAs. Thus, each receptor is independently capable of coupling with mitogenic signal-transduction pathways inherently present in these cells. Activation of either receptor also resulted in chemotaxis, alterations in inositol lipid metabolism, and mobilization of intracellular Ca2+. The magnitude of these functional responses correlated well with the binding properties of the different PDGF isoforms to each receptor. Thus, availability of specific PDGF isoforms and relative expression of each PDGF-receptor gene product are major determinants of the spectrum of known PDGF responses.

Original languageEnglish
Pages (from-to)8314-8318
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number21
DOIs
StatePublished - 1989
Externally publishedYes

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